Protein Acetylation: Methods and Protocols

ISBN-13: 9781493994335 / Angielski / Twarda / 2019 / 310 str.

Robert Brosh Jr

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Detection, Quantification, and Isolation of Acetylated Protein Targets

1. High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets

Birgit Schilling, Jesse G. Meyer, Lei Wei, Melanie Ott, and Eric Verdin

2. Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry

Paula A. Agudelo Garcia, Miranda Gardner, Michael A. Freitas, and Mark R. Parthun

3. Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty

Pavlo Lazarchuk, Sunetra Roy, Katharina Schlacher, and Julia Sidorova

Part II Lysine Acetylation

4. Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC

Catherine W. Njeri, Onyekachi E. Ononye, and Lata Balakrishnan

5. Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo

Anahita Lashgari, Jean-Philippe Lambert, and Jacques Côté

6. Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments

Anastasia J. Lindahl, Alexis J. Lawton, Josue Baeza, James A. Dowell, and John M. Denu

7. Lysine Acetylation of Proteins and its Characterization in Human Systems

David K. Orren and Amrita Machwe

Part III Sirtuin Targets of Acetylation

8. Molecular and Cellular Characterization of SIRT1 Allosteric Activators

Michael B. Schultz, Conrad Rinaldi, Yuancheng Lu, João A. Amorim, and David A. Sinclair

9. Assessment of SIRT2 Inhibitors in Mouse Models of Cancer

Yashira L. Negrón Abril, Irma Fernández, and Robert S. Weiss

Part IV Modulation of DNA Repair by Acetylation

10. Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair 

Amelia J. Hodges, Steven A. Roberts, and John J. Wyrick

11. Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes

Shrabasti Roychoudhury, Suravi Pramanik, Hannah L. Harris, and Kishor K. Bhakat

12. Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation

Onyekachi E. Ononye, Catherine W. Njeri, and Lata Balakrishnan

13. Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons

Ping-Chieh Pao, Jay Penney, and Li-Huei Tsai

Part V DNA Interactive Proteins and Processing Enzymes Affected by Acetylation

14. Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability

Chenlin Song and Ingrid Grummt

15. Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling 

Marina Farkas and Steven B. McMahon

Part VI Influence of Acetylation Processes on Neuroepigenetics and Tumorigenesis

16. Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics

Craig Myrum and Peter R. Rapp

17. Functional Analysis of HDACs in Tumorigeneis

Melissa Hadley, Satish Noonepalle, Debarati Banik, and Alejandro Villagra

Blurb

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.