Principles and Reactions of Protein Extraction, Purification, and Characterization

ISBN-13: 9781138406971 / Angielski / Twarda / 2017 / 408 str.

Ph.D. (University of Maryland Biotechnology Ins Hafiz Ahmed;Hafiz Ahmed PhD (University of Maryland Biotechnology Instit

Opis

Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations, diagrams, and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method. Presenting the advantages and disadvantages of the various protein techniques, Principles and Reactions of Protein Extraction, Purification, and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.

Recenzje

"Organized in a logical fashion…the most positive features in each chapter are the occasional placement of actual laboratory procedures (called "working procedures") and discussions on the advantages and disadvantages of each technique/method, often presented in tabular format. This comparison of methods and advice on when certain methods should be used is perhaps the most valuable feature of the book."
- Biochemistry and Molecular Biology Education

Spis treści

Chapter 1 Extraction of Protein, Chapter 2 Estimation of Protein, Chapter 3 Electrophoretic Analyses of Protein, Chapter 4 Purification of Protein, Chapter 5 Antibodies: Structures, Interactions, and Production, Chapter 6 Antibody Labeling, Antibody Detection, and Immunochemical Techniques, Chapter 7 Purification of Glycoproteins and Analyses of their Oligosaccharides, Index

O autorze

Hafiz Ahmed PhD, Hafiz Ahmed