Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing

ISBN-13: 9781071602737 / Angielski / Twarda / 2020 / 344 str.

Hirak Ranjan Dash; Pankaj Shrivastava; Surajit Das

Spis treści

Module 1: Overview of Forensic DNA analysis.- Chapter1:  Introduction to Forensic DNA analysis.- Chapter 2: Biological samples: the source targets for DNA typing.- Chapter 3: Collection, transportation and preservation and of biological evidences for DNA analysis.- Module2: Manual Extraction of DNA from biological samples.- Chapter 4: Manual Isolation of DNA from whole blood.- Chapter 5. Manual Isolation of DNA from body fluid stains.- Chapter 6: Manual isolation of DNA from soft tissue sample.- Chapter7: Manual isolation of DNA from rooted hair samples.- Chapter 8: Manual Isolation of DNA from human teeth.- Chapter 9: Isolation of DNA from bone samples.- Chapter  10:           Differential extraction of sperm and epithelial cell DNA.- Module 3: Automatic and semi-automatic extraction of DNA from biological samples.- Chapter 11: Isolation of DNA by using magnetic bead based extraction system.- Chapter 12: Extraction of DNA by using anion exchange resin Chelex®.- Chapter 13: Isolation of DNA by using column based extraction system.- Chapter 14. Reliable use of Whatman™ FTA™ cards for one step collection and isolation of DNA.- Module 4: Qualitative and quantitative assessment of extracted DNA.- Chapter 15: Quantification of DNA by using agarose gel electrophoresis technique.- Chapter 16: Quantification of DNA by using UV-Visible spectrophotometer.- Chapter 17: Quantification of DNA by using qRT-PCR.- Chapter 18: Fluorescence based instant quantification of DNA.- Module 5: Amplification of various STR markers using multiplex PCR.- Chapter 19: Amplification of autosomal chromosome STR markers by multiplex PCR.- Chapter 20: Amplification of Y chromosome STR markers by multiplex PCR.- Chapter 21: Amplification of X chromosome STR markers by multiplex PCR.- Chapter 22: Amplification of Autosomal Mini STR markers from degraded samples.- Module 6. Genotyping and analysis of Results.- Chapter 23:  Separation of amplified DNA fragments by capillary electrophoresis using Genetic Analyzer 3500xL.- Chapter 24:  Analysis of capillary electrophoresis results by GeneMapper® ID-X v 1.5 software.- Chapter 25: Calculation of paternity index in paternity dispute and identification cases.- Module 7: Case studies.- Chapter 26: DNA analysis of a disputed paternity caseSolving paternity dispute by DNA fingerprinting: A case study.- Chapter 27: Solving a case of murder by DNA fingerprinting: A case study.- Chapter 28: Solving a case of sexual assault by DNA fingerprinting: A case study.- Chapter 29: Identification of an unknown skeleton by DNA fingerprinting: A case study.- Module 8. Advanced technologies for forensic DNA analysis.- Chapter 30: Sequencing control region of human mitochondrial genome to assess matrilineal lineage.- Chapter 31: Application of Next Generation Sequencing (NGS) in forensic DNA analysis and DNA Phenotyping.- Chapter 32: Forensic Trace and Touch DNA analysis.- Chapter 33: RAPID DNA technology: A boon to forensic DNA typing.      

 

O autorze

Dr. Hirak Ranjan Dash is currently a Scientific Officer at the DNA Fingerprinting Unit, Forensic Science Laboratory, in Sagar, Madhya Pradesh, India. He completed his Ph.D. at the National Institute of Technology, Rourkela, India in 2015, and has since been working in the area of microbial ecology, forensic microbiology, and human genetics. In addition, he is a member of various scientific societies, including the International Society for Forensic Genetics, Association of Microbiologists of India and Students Association of Microbiology. He has been engaged in forensic DNA fingerprinting examinations in criminal and civil cases including the cases related to paternity, child swapping, rape, identity and incest. He has authored more than twenty five papers in reputed peer-reviewed international journals and has published four books.

Dr. Pankaj Shrivastava has broad interests in several areas of forensic DNA fingerprinting including population-based DNA analysis, developing protocols in forensic DNA typing, validating new multiplex kits and optimizing different steps in forensic DNA typing methodology. He has supervised Ph.D. students and published over 40 research papers in peer-reviewed international journals. His publications in Forensic Science International Genetics, Nature Scientific Reports, Legal Medicine, International Journal of Legal Medicine, Annals in human Biology etc., have earned him wide acclaim from the forensic DNA community. He is presently working at the DNA Fingerprinting Unit in State Forensic Science Laboratory, Govt. of Madhya Pradesh, India. He is a member of International Society for Forensic Genetics (ISFG), Indian Society of Human Genetics (ISHG), Association of Microbiologists of India(AMI) and Association for the promotion of DNA Fingerprinting and other DNATechnologies (ADNAT).

Dr. Surajit Das is an Associate Professor at the Department of Life Science at the National Institute of Technology, Rourkela, India. His field of research includes microbial genomics, biosynthesized nanoscale materials, marine biotechnology, environmental microbiology and bioremediation. He is serving as the Editor of Plos One and BMC Microbiology journal. He has published more than 70 research papers in peer-reviewed national and international journals and authored 7 books.         

Blurb

The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples.

It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples.

Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis.

Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.