Practical Protein Chromatography

ISBN-13: 9781489940162 / Angielski / Miękka / 2013 / 327 str.

Andrew Kenney; Susan Fowell

Opis

0ne 0fthe many 1mpact5 0frec0m61nant DNA techn0109y 0ver the 1a5t 15 year5 ha5 6een a 5tr0n91y refre5hed 1ntere5t 1n meth0d5 f0r the 5eparat10n and pur1f1cat10n 0f pr0te1n5.7h15 1ntere5t ha5 enc- pa55ed n0t 0n1y ana1yt1ca1 5eparat10n5, 6ut a150 5ma11- and 1ar9e-5ca1e preparat1ve meth0d5 d1rected t0 60th pure and app11ed re5earch thr0u9h0ut 610109y and 610med1c1ne. Many 0f the new 0r 5u65tant1a11y m0d1f1ed techn14ue5 deve1- 0ped have 6een rep0rted 1n the 11terature, 6ut a 5uff1c1ency 0f deta11ed pract1ca1 he1p 1n e5ta6115h1n9 the5e meth0d5 f0r the f1r5t t1me 1n a new 1a60rat0ry ha5 0ften 6een d1ff1cu1t t0 f1nd. W1th the5e pr061em5 1n m1nd, we expect that Pract1ca1Pr0te1n Chr0mat09raphy, de519ned a5 a key v01ume 1n the Meth0d5 1n M01ecu1ar 810109y 5er1e5, w111 pr0v1de c0nc15e pract1ca1 he1p t0 th05e carry1n9 0ut new techn14ue5 f0r the f1r5t t1me. Each chapter ha5 6een wr1tten 6y expert auth0r5 kn0wn t0 have d1rect and re9u1ar pract1ca1 exper1ence w1th the1r ch05en techn14ue5. 7he 5tructure 0feach chapter 15 de519ned t0 make 1t ea5y f0r a w0rker new t0 the meth0d t0 f0110w 1t t0 an effect1ve c0nc1u510n. An 1nt- duct10n treat5 the the0ry 6eh1nd the meth0d 6e1n9 de5cr16ed. 7he Mater1a15 and Meth0d5 5ect10n5 a110w the reader t0 prepare f0r, and then perf0rm techn14ue5 1n a rat10na1 5tepw15e manner. 7he N0te5 5ect10n5 pr0v1de the 50rt 0f6ack9r0und h1nt5 and tr1ck5 that are 50 0ften e55ent1a1 f0r 5ucce55, 6ut are rare1y rep0rted 1n the 11terature.

Recenzje

...an indispensable, hands-on guide... -Unlisted Drugs

Spis treści

Thiophilic Adsorption Chromatography. Immobilized Metal Ion Affinity Chromatography. Histidine Ligand Affinity Chromatography. Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes. Calcium-Dependent Hydrophobic Interaction Chromatography. Lectin Affinity Chromatography. Dye-Ligand Affinity Chromatography. High-Performance Affinity Chromatography for Protein Separation and Purification. Immunoaffinity Chromatography. Selection for Antibodies for Immunoaffinity Chromatography. Some Alternative Coupling Chemistries for Affinity Chromatography. Exploiting Weak Affinities. Biospecific Affinity Elution. Size-Exclusion High-Performance Liquid Chromatography of Proteins. Chromatofocusing. Ion-Exchange Chromatography of Proteins. Displacement Chromatography of Proteins. Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography. Making Bacterial Extracts Suitable for Chromatography. Determination of Purity and Yield. Index.