Practical HPLC Method Development

ISBN-13: 9780471007036 / Angielski / Twarda / 1997 / 800 str.

Lloyd Snyder; Joseph Kirkland; Joseph Glajch

Opis

High Performance Liquid Chromatography (HPLC) is a common method for analyzing substances and is used by researchers to measure the amount of caffeine in coffee, for example, or the level of penicillin in a blood sample. The process involves separating a substance into its individual components and then examining the result.

Spis treści

Getting Started.

Basics of Separation.

Detection Sensitivity and Selectivity.

Sample Preparation.

The Column.

Non–Ionic Samples: Reversed– and Normal–Phase HPLC.

Ionic Samples: Reversed–Phase, Ion–Pair, and Ion–Exchange HPLC.

Gradient Elution.

Systematic Approach to the Reversed–Phase Separation of Regular Samples.

Computer–Assisted Method Development.

Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

Chiral Separations.

Preparative HPLC Separation.

Quantitation (Including Trace Analysis).

Completing the Method: Validation and Transfer.

Appendices.

Index.