Population Epigenetics: Methods and Protocols

ISBN-13: 9781493969012 / Angielski / Twarda / 2017 / 206 str.

Paul Haggarty; Kristina Harrison

Opis

This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions.

Spis treści

1. Library Construction for High-Throughput Mobile Element Identification and Genotyping

Hongseok Ha, Nan Wang, and Jinchuan Xing

 

2. The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements

Gwen Hoad and Kristina Harrison

 

3. Determining Epigenetic Targets: A Beginner’s Guide to Identifying Genome Functionality Through Database Analysis

Elizabeth A. Hay, Philip Cowie, and Alasdair MacKenzie

 

4. Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C

Matteo Vietri Rudan, Suzana Hadjur, and Tom Sexton

 

5. Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method

Vuthy Ea, Franck Court, and Thierry Forne

 

6. 5-Hydroxymethylcytosine Profiling in Human DNA

John P. Thomson, Colm E. Nestor, and Richard R. Meehan

 

7. Adjusting for Cell Type Composition in DNA Methylation Data Using a Regression-Based Approach

Meaghan J. Jones, Sumaiya A. Islam, Rachel D. Edgar, and Michael S. Kobor

 

8. Correcting for Sample Heterogeneity in Methylome-Wide Association Studies

James Y. Zou

 

9. Nano-MeDIP-seq Methylome Analysis Using Low DNA Concentrations

Lee M. Butcher and Stephan Beck

 

10. Bisulfite Conversion of DNA from Tissues, Cell Lines, Buffy Coat, FFPE Tissues, Microdissected Cells, Swabs, Sputum, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine

Maria Jung, Barbara Uhl, Glen Kristiansen, and Dimo Dietrich

 

11. Analysis of Imprinted Gene Regulation

David A. Skaar and Randy L. Jirtle

 

12. Statistical Methods for Methylation Data

Graham W Horgan and Sok-Peng Chua

Blurb

This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity.