Polypeptide Materials: Methods and Protocols

ISBN-13: 9781071609279 / Angielski / Twarda / 2020 / 268 str.

Maxim G. Ryadnov

Spis treści

1. A Shortcut to the Synthesis of Peptide Thioesters Using an HF-Free Approach

            Richard Raz and John Offer

 

2. SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification

            Can M. Buldun, Irsyad N.A. Khairil Anuar, and Mark Howarth

 

3. Peptide Nanoparticles for Gene Packaging and Intracellular Delivery

            Paula V. Gomez, James E. Noble, and Maxim G. Ryadnov

 

4. Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung

            Jade J. Welch, David A. Dean, and Bradley L. Nilsson

 

5. FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures

            Simon Chi-Chin Shiu, Yusuke Sakai, Julian A. Tanner, and Jonathan G. Heddle

 

6. Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes

            Irene Marzuoli and Franca Fraternali

 

7. Functional Peptide Nanocapsules Self-Assembled from b-Annulus Peptides

            Hiroshi Inaba and Kazunori Matsuura

 

8. Electrostatic Self-Assembly of Protein Cage Arrays

            Soumyananda Chakraborti, Antti Korpi, Jonathan G. Heddle, and Mauri A. Kostiainen

 

9. Design and Generation of Self-Assembling Peptide Virus-Like Particles with Intrinsic GPCR Inhibitory Activity

            Sergey G. Tarasov, Marzena Dyba, Joshua Yu, and Nadya Tarasova

 

10. Imaging and 3D Reconstruction of De Novo Peptide Capsids

            Emiliana De Santis and Maxim G. Ryadnov

 

11. Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra

            Abeer Alghamdi, Li Hung C. Chung, and Olaf Rolinski

 

12. Forming Low Molecular Weight Hydrogels by Electrochemical Methods

            Emily R. Cross, Kate McAulay, and Dave J. Adams

 

13. In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study

            Tristan O.C. Kwan, Rosana Inácio dos Reis, and Isabel Moraes

 

14. Measurement of Peptide Coating Thickness and Chemical Composition Using XPS

            David J. H. Cant, Alexander G. Shard, and Caterina Minelli

 

15. Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy

            Katharine Hammond, Georgina Benn, Isabel Bennett, Edward S. Parsons, Maxim G. Ryadnov, Bart W. Hoogenboom, and Alice L.B. Pyne

 

16. Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials

            Jehangir Cama and Stefano Pagliara

 

17. Ultramicrotomy Analysis of Peptide-Treated Cells

            Stephanie Rey, Nilofar Faruqui, and Maxim G. Ryadnov

Blurb

This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.