Platelet Proteomics

ISBN-13: 9780470463376 / Angielski / Twarda / 2011 / 432 str.

Angel Garcia

Opis

The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.

Recenzje

"Experienced researchers looking for an up–to–date and comprehensive review of this rapidly developing field will find this book a valuable resource.   (American Society for Mass Spectrometry, 13 December 2011)

Spis treści

Contributors.

Foreword.

Preface.

Acronyms.

PART I GENERAL OVERVIEW: PLATELETS, SAMPLE PREPARATION, AND MASS SPECTROMETRY–BASED PROTEOMICS.

1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis (Ronald G. Stanley, Katherine L. Tucker, Natasha E. Barrett, and Jonathan M. Gibbins).

2 Mass–Spectrometry–Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research (Julia Maria Burkhart and Albert Sickmann).

3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research (Maria Zellner and Rudolf Oehler).

PART II ANALYSIS OF THE PLATELET PROTEOME: GLOBAL APPROACHES AND SUBPROTEOMES.

4 Two–Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies (Angel Garcia).

5 The Platelet Membrane Proteome (Yotis A. Senis).

6 Proteomics of Platelet Granules, Organelles, and Releasate (James P. McRedmond).

7 The Platelet Microparticle Proteome (David M. Smalley).

8 N–Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome (Francis Impens, Kenny Helsens, Niklaas Colaert, Lennart Martens, Joel Vandekerckhove, and Kris Gevaert).

PART III INTEGRATED "OMICS" AND APPLICATION TO DISEASE.

9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome (Michael G. Tomlinson).

10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet–Associated Disorders (Dmitri V. Gnatenko and Wadie F. Bahou).

11 Platelet Functional Genomics (Isabelle I. Salles, Marie N. O Connor, Daphne C. Thijssen–Timmer, Katleen Broos, and Hans Deckmyn).

12 Systems Biology to Study Platelet–Related Bleeding Disorders (Jan–Willem N. Akkerman and Bernard De Bono).

13 Platelet Proteomics in Transfusion Medicine (Thomas Thiele, Leif Steil, Uwe V¨olker, and Andreas Greinacher).

14 Cardiovascular Proteomics (Fernando Vivanco, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, and Gloria A´lvarez–Llamas).

Index.

O autorze

Dr. Ángel García is currently a Ramón y Cajal Research Fellow at the Pharmacology Department, Universidade de Santiago de Compostela, Spain His main research is the area of platelet proteomics, with special emphasis in cardiovascular research and platelet signaling studies. He has written over twenty publications in peer–reviewed journals and has been an invited lecturer at the Platelets 2010 International Symposium (Jerusalem, Israel), and the XXII Congress of the International Society on Thrombosis and Haemostasis, held in Boston, United States, in 2009, where he chaired a session on novel approaches to understanding platelet biology. Sr. Garcia was a postdoctoral research associate of the Proteomics Group at the Oxford Glycobiology Institute, University of Oxford ((UK), from 2001 to 2005.

Dr. Yotis Senis  is currently a British Heart Foundation Research Fellow at the University of Birmingham, United Kingdom. He is a senior member of the Birmingham Platelet Group. Dr. Senis research interests include the identification of novel platelet surface glycoproteins and determining their biological and molecular functions. He is also interested in the functional roles of protein tyrosine phosphatases in platelets and thrombosis. Dr. Senis has been an invited lecturer at the 2010 International Symposium (Jerusalem, Israel) and the Gordon Research Conference on the Cell Biology of Megakaryocytes and Platelets (Galveston, Texas). He has written over twenty publications on platelet signaling and thrombosis.

Blurb

The groundbreaking new book that covers platelets, their role, and their study through proteomics research

Platelets are defined as small anucleate cells that circulate in the blood and play a fundamental role in haemostasis and thrombosis. From a pharmacological perspective, unwanted platelet activation forms blood clots and promotes cardiovascular disease–the leading cause of death in the Western world.

Platelet Proteomics focuses on the cutting–edge science in the emerging field of platelet proteomics research–a vital analytical method for the study of platelet activity. Written by a variety of contributions at the forefront of platelet research, this invaluable reference covers the recent developments in analyzing the proteome of basal and activating platelets in providing opportunities for advancing the discovery of new proteins that could eventually become drug targets for platelet–based illness. Features of this book include:

• Coverage of non–gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel–based separation methods (2–DE), and sub–proteome analyses (secretome/releasate, membrane proteins, organelles)
• A focus on the main research done on platelet proteomics, such as general proteome mapping, mapping signaling pathways, secretome and membrane proteomics, studies integrating other –omics, and application to platelet–related diseases
• An examination of platelet–specific problems in a widely applicable fashion–the signaling proteome chapter serves as a model for investigation in many other cell types.

Covering key principles as well as the latest applications in proteome analyses, Platelet Proteomics makes a significant contribution to an area of study with exciting potential. This inaugural foray into platelet exploration is a blueprint for the future and is expansive enough in scope to reach across many scientific disciplines.