Plasmids: Biology and Impact in Biotechnology and Discovery

ISBN-13: 9781555818975 / Angielski / Twarda / 2015 / 718 str.

Marcelo E. Tolmasky; Juan Carlos Alonso

Opis

Explore the remarkable discoveries in the rapidly expanding field of plasmid biology Plasmids are integral to biological research as models for innumerable mechanisms of living cells, as tools for creating the most diverse therapies, and as crucial helpers for understanding the dissemination of microbial populations. Their role in virulence and antibiotic resistance, together with the generalization of "omics" disciplines, has recently ignited a new wave of interest in plasmids. This comprehensive book contains a series of expertly written chapters focused on plasmid biology, mechanistic details of plasmid function, and the increased utilization of plasmids in biotechnology and pharmacology that has occurred in the past decade. Plasmids: Biology and Impact in Biotechnology and Discovery serves as an invaluable reference for researchers in the wide range of fields and disciplines that utilize plasmids and can also be used as a textbook for upper-level undergraduate and graduate courses in biotechnology and molecular biology.

Spis treści

Contributors Preface I. Introduction 1. Historical Events That Spawned the Field of Plasmid Biology II. Plasmid Replication Systems and Their Control 2. Iteron Plasmids 3. Mechanisms of Theta Plasmid Replication 4. Plasmid Rolling-Circle Replication 5. Replication and Maintenance of Linear Phage-Plasmid N15 6. Plasmid Replication Control by Antisense RNAs 7. Topological Behavior of Plasmid DNA III. Plasmid Maintenance, Transfer and Barriers 8. Plasmid Partition Mechanisms 9. Resolution of Multimeric Forms of Circular Plasmids and Chromosomes 10. Conditional Activation of Toxin-Antitoxin Systems: Postsegregational Killing and Beyond 11. The Interplay between Different Stability Systems Contributes to Faithful Segregation: Streptococcus pyogenes pSM19035 as a Model 12. The CRISPR-Cas Immune System and Genetic Transfers: Reaching an Equilibrium 13. Plasmid Diversity and Adaptation Analyzed by Massive Sequencing of Escherichia coli Plasmids 14. Conjugation in Gram-Positive Bacteria 15. Mobilizable Rolling-Circle Replicating Plasmids from Gram-Positive Bacteria: A Low-Cost Conjugative Transfer IV. Specific Plasmid Systems 16. The Plasmid Mobilome of the Model Plant Symbiont Sinorhizobium meliloti: Coming up with New Questions and Answers 17. The Agrobacterium Ti Plasmids 18. The Influence of Biofilms in the Biology of Plasmids 19. The Partitioning and Copy Number Control Systems of the Selfish Yeast Plasmid: An Optimized Molecular Design for Stable Persistence in Host Cells 20. Plasmids from Euryarchaeota V. Plasmid Ecology and Evolution 21. The Plasmidome of Firmicutes: Impact on the Emergence and the Spread of Resistance to Antimicrobials 22. Plasmid-Mediated Antimicrobial Resistance in Staphylococci and Other Firmicutes 23. Plasmid Detection, Characterization, and Ecology 24. Plasmid-Mediated Antibiotic Resistance and Virulence in Gram-Negatives: The Klebsiella pneumoniae Paradigm 25. Plasmid-Mediated Quinolone Resistance VI. Specialized Functions Mediated By Plasmids 26. Plasmid-Mediated Tolerance Toward Environmental Pollutants 27. Virulence Plasmids of Spore-Forming Bacteria 28. Virulence Plasmids of Nonsporulating Gram-Positive Pathogens 29. Plasmid-Encoded Iron Uptake Systems VII. Plasmids as Genetic Tools 30. DNA Assembly Tools and Strategies for the Generation of Plasmids 31. Plasmids as Tools for Containment 32. Mining Environmental Plasmids for Synthetic Biology Parts and Devices 33. Using Plasmids as DNA Vaccines for Infectious Diseases 34. Plasmid Biopharmaceuticals Index