ISBN-13: 9783659576928 / Angielski / Miękka / 2014 / 120 str.
Viruses appear to be the most destructive group of plant pathogens. Cloning in plasmid vectors has a wide range of practical applications in plant molecular biology.This investigation was curried out to achieve:1- Conducting some molecular studies on plant viruses such as: Extraction of RNA, Reverse transcriptiom reaction, PCR, Cloning of PCR product, Subcloning. Using of modern serological methods for rapid detection of plant viruses such as: ELISA, DBIA, and TBIA. In addition to achieve ultrathin sectioning using electron microscopy to recognize the internal changes induced by virus, and abnormalities caused by virus using light microscopy.
Viruses appear to be the most destructive group of plant pathogens. Cloning in plasmid vectors has a wide range of practical applications in plant molecular biology.This investigation was curried out to achieve :1- Conducting some molecular studies on plant viruses such as : Extraction of RNA, Reverse transcriptiom reaction, PCR, Cloning of PCR product, Subcloning. Using of modern serological methods for rapid detection of plant viruses such as: ELISA , DBIA, and TBIA. In addition to achieve ultrathin sectioning using electron microscopy to recognize the internal changes induced by virus, and abnormalities caused by virus using light microscopy.