Plant Organogenesis: Methods and Protocols

ISBN-13: 9781493959310 / Angielski / Miękka / 2016 / 356 str.

Ive De Smet

Opis

This Methods in Molecular Biology (TM) book covers topics such as how to image the structure of plant ovules and embryos, tools for establishing cell lineages, methods for studying the totipotency of plant cells, fluorescence-activated cell sorting and more.

Spis treści

1. The Tomato Leaf as a Model System for Organogenesis

            Yogev Burko and Naomi Ori

 

2. Usefulness of Physcomitrella patens for Studying Plant Organogenesis

            Sandrine Bonhomme, Fabien Nogué, Catherine Rameau, and Didier G. Schaefer

 

3. The Dicot Root as a Model System for Studying Organogenesis

            Julie Lavenus, Michael Lucas, Laurent Laplaze, and Soazig Guyomarc’h

 

4. Genetic Control of Root Organogenesis in Cereals

            Caroline Marcon, Anja Paschold, and Frank Hochholdinger

 

5. Gene Expression Analysis of Aquatic Angiosperms Podostemaceae to Gain Insight into the Evolution of Their Enigmatic Morphology

            Satoshi Koi and Natsu Katayama

 

6. Brown Algae as a Model for Plant Organogenesis

            Kenny A. Bogaert, Alok Arun, Susana M. Coelho, and Olivier De Clerck

 

7. Microscopic Analysis of Ovule Development in Arabidopsis thaliana

            Balaji Enugutti, Maxi Oelschner, and Kay Schneitz

 

8. Imaging of Phenotypes, Gene Expression and Protein Localization During Embryonic Root Formation in Arabidopsis

            Cristina Llavata-Peris, Annemarie Lokerse, Barbara Möller, Bert De Rybel, and Dolf Weijers

 

9. Inducible System for Lateral Roots in Arabidopsis thaliana and Maize

            Leen Jansen, Boris Parizot, and Tom Beeckman

 

10. Adventitious Root Induction in Arabidopsis thaliana as a Model for in vitro Root Organogenesis

            Inge Verstraeten, Tom Beeckman, and Danny Geelen

 

11. High-Throughput, Non-Invasive Imaging of Root Systems

            Anjali S. Iyer-Pascuzzi, Paul R. Zurek, and Philip N. Benfey

 

12. Small Molecule Screens to Study Lateral Root Development

            Dominique Audenaert, Bert De Rybel, Long Nguyen, and Tom Beeckman

 

13. Cell Lineage Analyses in Living Tissues

            John Runions and Smita Kurup

 

14. Protein Immunolocalization in Maize Tissues

            Cristian Forestan, Nicola Carraro, and Serena Varotto

 

15. Auxin Immunolocalization in Plant Tissues

            Cristian Forestan and Serena Varotto

 

16. Gene Expression Analysis of Shoot Apical Meristem Cell Types

            Ram Yishor Yadav, Stephen Snipes, Thomas Girke, and G. Venugopala Reddy

 

17. Kinematic Analysis of Cell Division in Leaves of Mono- and Dicotyledonous Species: A Basis for Understanding Growth and Developing Refined Molecular Sampling Strategies

            Hilde Nelissen, Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabuo Fiorani, and Gerrit T.S. Beemster

 

18. Regeneration in Arabidopsis Tissue Culture

            Kaoru Sugimoto and Elliot M. Meyerowitz

 

19. Isolation and Analysis of mRNAs from Specific Cell Types of Plants by Ribosome Immunopurification

            Angelika Mustroph, Eugenia Zanetti, Thomas Girke, and Julia Bailey-Serres

 

20. Analyzing Small and Long RNAs in Plant Development Using Non-Radioactive in-situ Hybridization

            Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, and Martin Crespi

 

21. Analyzing Protein Distribution in Plant Tissues Using “Whole-Mount” Immunolocalization

            Pilar Bustons-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, and Martin Crespi

 

22. Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus

            Aude Le Bail and Bénédicte Charrier

 

23. Building Simulation Models of Developing Plant Organs Using VirtualLeaf

            Roeland M.H. Merks and Michael Guravage

Blurb

Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating research field.