Plant Molecular Biology Manual

ISBN-13: 9789401176552 / Angielski / Miękka / 2012 / 161 str.

S. B. Gelvin;R. a. Schilperoort

Opis

Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.

Recenzje

About the first edition:
` Altogether, Plant Molecular Biology Manual is a very useful guide for the practical application of modern methods of molecular biology in plant sciences and is therefore highly recommended. '
Plant Breeding
` ...every researcher in plant molecular biology should take a look at this book '
New Phytologist

Spis treści

Supplement I: F4. Polymerase chain reaction: gene detection, inverse PCR, and genetic engineering; J. Slightom, et al. G3. Light microscopic applications of immunocytochemistry; K. Vandenbosch, et al. K3. T-DNA tagging in Arabidopsis thaliana: Cloning by gene disruption; B. Schulz, et al. L1. High level gene expression in plants using RNA viruses as transient expression vectors; C.M. Kearney, et al. L2. Geminivirus mediated gene transfer; D. Bisaro, G. Sunter. L3. Caulimovirus mediated gene transfer; I. Jupin, B. Gronenborn. Supplement II: RNA differential display; M.J. Laskowski. Expression analysis of multigene Families by RFLP-coupled domain-directed differential display (RC4D); A. Fischer, et al. Recombinant aequorin methods for measurement of intracellular calcium in plants; H. Knight, et al. GUS activity staining - A powerful tool in plant molecular biology; Jeong Hee Lee, F. Schöffl. In-Cell RT-PCR in a single, detached plant cell; Ho-Hyung Woo. Supplement III: A3. Liposome-mediated transfer of YAC-DNA to tobacco cells; M. Van Wordragen, et al. B7. Agribacterium germ-line transformation: Transformation of Arabidopsis without tissue culture; A.F. Bent, S. Clough. D6. RNA stability of chloroplasts; J. Nickelsen. G5. Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH); P. Fransz, et al. L4. Tobamovirus transient expression vectors: Tools for plant biology and high-level expression of foreign proteins in plants; G.P.pogue, et al. M1. Identification and characterization of protein interactions using the yeast 2-hybrid system; S.E. Kohalmi, et al. Index. Supplement IV: List of Chapters. The construction of bacterial artificial chromosome (BAC) libraries; S. Choi, R.A. Wing. Yeast as a versatile tool in transcription factor research; A.H. Meijer, et al. Gene transfer mediated by site-specific recombination systems; L.A. Lyznik, et al. Use of the R/RS site-specific recombination system in plants; H. Albert, et al. Cre/Lox directed integration of transgenes into the tobacco genome; H. Albert, D.W. Ow. Plant actin isolation and characterization; C.J. Staiger, et al. Basic Work: Section A: In vitro methods of gene transfer to plant cells. 1. PEG-mediated direct gene transfer and electroporation; R. Bilang, A. Klöti, M. Schrott, I. Potrykus. 2. Gene transfer to plants via particle bombardment; P. Christou. Section B: Agrobacterium-mediated gene transfer to plant cells. 1. Agrobacterium-mediated gene transfer to plant cells: Cointegrate and binary vectors; C.R. Walkerpeach, J. Velten. 2. Specialized vectors for gene tagging and expression studies; C. Koncz, N. Martini, L. Szabados, M. Hrouda, A. Bachmair, J. Schell. 3. Agrobacterium molecular genetics; P.J.J. Hooykaas. 4. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species; S.B. Gelvin, Chang-Nong Liu. 5. Transient expression assays using GUS constructs and fluorometric detection for