Plant Lipids: Methods and Protocols

ISBN-13: 9781071613610 / Angielski / Twarda / 2021 / 472 str.

Dorothea Bartels; Peter Dörmann

Spis treści

Preface…
Table of Contents…
Contributing Authors…

Part I Basic Methods of Lipid Isolation and Analysis

1 Lipid Isolation from Plants
Jesse D. Bengtsson, James G. Wallis, and John Browse

2. Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and Yield
Samira Mahboub, Zachery D. Shomo, R. Maxwell Regester, Mahaa Albusharif, and Rebecca L. Roston

3. Thin-Layer Chromatography 
Georg Hölzl and Peter Dörmann

4. Lipid Analysis by Gas Chromatography and Gas Chromatography Mass Spectrometry
Mathias Brands, Philipp Gutbrod, and Peter Dörmann

5. 14C-Tracing of Lipid Metabolism
Hari Kiran Kotapati and Philip D. Bates

6. Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes
Masako Iwai, Shiori Shibata, Hiroyuki Ohta, and Koichiro Awai

Part II Mass Spectrometry and NMR Analysis

7. Direct Infusion Mass Spectrometry for Complex Lipid Analysis 
Katharina Gutbrod, Helga Peisker, and Peter Dörmann

8. Fatty Acid Composition by Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight (TAL-CID-TOF) Mass Spectrometry
Pamela Tamura, Carl Fruehan, David K. Johnson, Paul Hinkes, Todd D. Williams, and Ruth Welti 

9. Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS
Cornelia Herrfurth, Yi-Tse Liu, and Ivo Feussner

10. Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism
Rebecca E. Cahoon, Ariadna Gonzalez Solis, Jonathan E. Markham, and Edgar B. Cahoon

11. Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MS
Richard Broughton and Frédéric Beaudoin

12. Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae
Richard P. Haslam and Tony R. Larson

13. Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS 
Lauren M. Jenkins, Jeong-Won Nam, Bradley S. Evans, and Doug K. Allen

14. Structural Analysis of Glycosylglycerolipids using NMR Spectroscopy
Wiebke Knaack, Georg Hölzl, and Nicolas Gisch

Part III Lipid Isolation and Analysis from Plant Tissues Cell Compartments and Organelles 

15. Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and Seeds
Johanna Baales, Viktoria V. Zeisler-Diehl, and Lukas Schreiber 

16. Isolation of Lipid Droplets for Protein and Lipid Analysis
Patrick J. Horn, Kent D. Chapman, and Till Ischebeck 

17. Isolation of Plastoglobules for Lipid Analyses
Denis Coulon and Claire Bréhélin

18. Isolation of Mitochondria for Lipid Analysis
Sébastien Leterme, Morgane Michaud, and Juliette Jouhet 

19. Collection and Analysis of Phloem Lipids 
Susanne Hoffmann-Benning

Part IV Lipid Signaling, Lipid-Protein Interactions, and Imaging

20. Analyses of Inositol Phosphates and Phosphoinositides by Strong Anion Exchange (SAX)-HPLC 
Debabrata Laha, Marília Kamleitner, Philipp Johnen, and Gabriel Schaaf

21. Analysis of Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption Chromatography and Subsequent Quantification via Thin-Layer and Gas Chromatography 
Larissa Launhardt, Monique Matzner, Mareike Heilmann, and Ingo Heilmann

22. Studying Lipid-Protein Interactions Using Protein-Lipid-Overlay and Protein-Liposome-Association Assays
Guido Ufer, Peter Dörmann, and Dorothea Bartels

23. Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC) 
Ze-Hua Guo and Mee-Len Chye

24. In Situ Localization of Plant Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI)
Drew Sturtevant, Mina Aziz, Trevor B. Romsdahl, Chase D. Corley, and Kent D. Chapman 

Part V Lipid Databases 

25. Plant Lipid Databases
Peter Dörmann 

26. Lipid Pathway Databases with a Focus on Algae 
Naoki Sato and Takeshi Obayashi

Subject Index List…

Blurb

This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally.