Plant Isoprenoids: Methods and Protocols

ISBN-13: 9781493953912 / Angielski / Miękka / 2016 / 303 str.

Manuel Rodriguez Concepcion

Opis

Plant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands as well as genetic, pharmacological and bioinformatic tools that are particularly useful for plant molecular biologists. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Plant Isoprenoids: Methods and Protocols will serve as an excellent reference material that can be adapted to develop customized methods for different needs making the world of plant isoprenoids more accessible for all researchers.

Spis treści

1. Plant Isoprenoids: A General Overview

Manuel Rodríguez-Concepción

 

I. Measurement of Core Enzyme Activities

 

2. Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts

Louwrance P. Wright and Michael A. Phillips

 

3. Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants

Narciso Campos, Montserrat Arró, Albert Ferrer, and Albert Boronat

 

4. Farnesyl Diphosphate Synthase Assay

Montserrat Arró, David Manzano, and Albert Ferrer

 

II. Targeted Analysis of Isoprenoid Metabolites

 

5. Metabolite Profiling of Plastidial Deoxyxylulose-5-Phosphate Pathway Intermediates by Liquid Chromatography and Mass Spectrometry

Edward E.K. Baidoo, Yanmei Xiao, Katayoon Dehesh, and Jay D. Keasling

 

6. Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity

Antonio J. Meléndez-Martínez, Carla M. Stinco, Paula Mapelli Brahm, and Isabel M. Vicario

 

7. Simultaneous Analyses of Oxidized and Reduced Forms of Photosynthetic Quinones by High-Performance Liquid Chromatography

Masaru Shibata and Hiroshi Shimada

 

8. Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry

Vera Wewer and Peter Dörmann

 

9. Analysis of Plant Polyisoprenoids

Katarzyna Gawarecka and Ewa Swiezewska

 

10. Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots

Qiang Wang, Reza Sohrabi, and Dorothea Tholl

 

11. Gas Chromatography Mass-Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants

Astrid Kännaste, Lucian Copolovici, and Ülo Niinemets

 

12. Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts using UPLC-qTOF Mass Spectrometry

Uwe Heinig and Asaph Aharoni

 

III. Isoprenoid Profiling in Specialized Organs

 

13. Isoprenoid and Metabolite Profiling of Plant Trichomes

Gerd Ulrich Balcke, Stefan Bennewitz, Sebastian Zabel, and Alain Tissier

 

14. Sample Preparation for Single Cell Transcriptomics - Essential Oil Glands in Citrus Fruit Peel as an Example

Siau Sie Voo and Bernd Markus Lange

 

15. Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions

Felix Kessler and Gaetan Glauser

 

16. Confocal Laser Scanning Microscopy Detection of Chlorophylls and Carotenoids in Chloroplasts and Chromoplasts of Tomato Fruit

Lucio D’Andrea, Montse Amenós, and Manuel Rodríguez-Concepción

 

IV. Genetic, Pharmacological, and Bioinformatic Tools

 

17. Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS

Ery O. Fukushima, Hikaru Seki, and Toshiya Muranaka

 

18. High Throughput Testing of Terpenoid Biosynthesis Candidate Genes Using Transient Expression in Nicotiana benthamiana

Søren Spanner Bach, Jean-Étienne Bassard, Johan Andersen-Ranberg, Morten Emil Møldrup, Henrik Toft Simonsen, and Björn Hamberger

 

19. Heterologous Stable Expression of Terpenoid Biosynthetic Genes Using the Moss Physcomitrella patens

Søren Spanner Bach, Brian Christopher King, Xin Zhan, Henrik Toft Simonsen, and Björn Hamberger

 

20. Quantification of Plant Resistance to Isoprenoid Biosynthesis Inhibitors

Catalina Perelló, Manuel Rodríguez-Concepción, and Pablo Pulido

 

21. A Flexible Protocol for Targeted Gene Co-Expression Network Analysis

Diana Coman, Philipp Rütimann, and Wilhelm Gruissem