Plant Hormones: Methods and Protocols

ISBN-13: 9781617379307 / Angielski / Miękka / 2011 / 146 str.

Sean Cutler; Dario Bonetta

Opis

The last 10 years have witnessed an explosion in our understanding of plant h- mones. The often vague models of hormone action developedover decadeshave been replaced in short order by detailed molecular models that include receptors and in many cases downstream signal transduction components. Given the rapid progress in understanding the mechanism of action of plant growth regulators, a technical review of hormone methodology is timely. Our book focuses on genetic, biochemical, ana- tical and chemical biological approaches for understanding and dissecting plant h- mone action. The greatest strides in plant hormone biology have come, by and large, from the use of genetic methods to identify receptors and we dedicate a chapter to general genetic methods of analysis using the model system Arabidopsis thaliana. A cluster of chapters focuses on biochemical methods for documenting interactions betweenhormonesand their receptors. Theimportance of these assays is tremendous; receptor-ligand interactions in animal model systems have been the cornerstones of pharmacological and medicinal chemical assays that have enabled identification of selective and non-selective agonists and antagonists that can be used to further probe and dissect questions of receptor function. This is likely to be a major new frontier in plant hormone research.

Recenzje

From the reviews of the second edition: "This book introduces several approaches essential for plant hormone biology at the forefront of research. ... This book has been published at an apt time for plant hormone biologists, especially for PhD students and advanced researchers in this field, to move the field to a higher stage and to lead them towards wider and more advanced technical approaches to hormone analyses, to encourage them to accumulate more specific genomic information and to apply more sophisticated analytical tools." (Tomokazu Koshiba, Annals of Botany, Vol. 105 (4), April, 2010)

Spis treści

Plant Hormones: Methods and Protocols, Second Edition S. Cutler and D. Bonetta, eds. Table of Contents Preface Contributors 1. Using reverse genetics to develop small knockout collections for specific biological questions. J. Northey and P. McCourt 2. Visualizing auxin transport routes in Arabidopsis leaf primordia. D. Marcos and T. Berleth 3. Bioinformatic analysis of hormone microarray data. G.W. Bassel and N.J. Provart 4. Measurement of plant hormones by liquid chromatography-mass spectrometry. S.J. Owen and S.R. Abrams 5. Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry. M. Okamoto, A Hanada, Y Kamiya, S Yamaguchi and E Nambara 6. Pull-down assays for plant hormone research. S. Kepinski 7. Binding assays for brassinosteroid receptors. A. Caño-Delgado and Z-Y Wang 8. Binding assays for abscisic acid receptors. F.A. Razem and R.D. Hill 9. Cytokinin sensing systems using microorganisms. M. Higuchi, T. Kakimoto and T. Mizuno 10. Hormone-binding assay using living bacteria expressing eukaryotic receptors. G.A. Romanov and S.N. Lomin 11. Discovery of plant microRNAs and short-interfering RNAs by deep parallel sequencing. P. Chellappan and H. Jin 12. The use of chemical genomics to investigate pathways intersecting auxin-dependent responses and endomembrane trafficking in Arabidopsis thaliana L. Norambuena, G.R. Hicks and N.V. Raikhel

Blurb

The last ten years have witnessed a monumental increase in our understanding of plant hormones. A decade ago, scientists were forced to rely on vague ideas about hormone action; today, these have been replaced by detailed molecular models. Given the rapid increase in our understanding of plant hormone biology, this comprehensive review could not have come at a better time. In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approaches for understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and cutting edge, Plant Hormones: Methods and Protocols, Second Edition is an essential guide for all plant scientists who are facing the complex and challenging questions that will determine the future of this vital field.