Plant Cytogenetics

ISBN-13: 9781439884188 / Angielski / Twarda / 2016 / 528 str.

Ram J. Singh

Opis

Cytogenetics plays an important role in understanding the chromosomal and genetic architecture of plant species. Plant Cytogenetics, Third Edition follows the tradition of its predecessors presenting theoretical and practical aspects of plant cytogenetics. Chapters describe correct handling of plant chromosomes, methods in plant cytogenetics, cell division, reproduction methods, chromosome nomenclature, karyotype analysis, chromosomal aberrations, genome analysis, transgenic crops, and cytogenetics in plant breeding.
This new edition begins with a brief introduction on the historical aspect of cytogenetics and flows directly into handling of plant chromosomes by classical and modern cytological techniques, classical Mendelian Genetics, brief description of cell division, and chromosome identification by karyotype analysis. The comprehension of cytogenetics is incomplete without information on the role of aneuploidy in associating a gene on a particular chromosome, and the book covers these methodologies as a primary topic. Covering classical to modern cytogenetics, the book presents to the reader the crucial role of cytogenetics in improving crops.

Spis treści

INTRODUCTION

THE HANDLING OF PLANT CHROMOSOMES
Introduction
Squash Technique - Mitotic and Meiotic Chromosomes
Smear Technique for Plant Chromosomes
Pollen Staining
Pollen-Stigma Incompatibility
Fluorescence in situ Hybridization (FISH)
Total DNA Extraction-Plant Genomic DNA
Determination of Nuclear DNA Content of Plants by Flow Cytometry
Karyotyping and Sorting of Plant Chromosomes by Flow Cytometry
Production of Wide Hybrids Through In Vitro Technique

CELL DIVISION
Introduction
Mitosis
Meiosis

GENETIC CONTROL OF MEIOSIS
Introduction
Synaptic Mutants
Genetic Control of Recombination
Genes Responsible for Chromosome Disjunction
Other Meiotic Mutants
Role of Heterochromatin in Chromosome Pairing
Diploid-like Meiosis in Allopolyploids
Haploidy
Male Sterility

MODE OF REPRODUCTION IN PLANTS
Introduction
Sexual Reproduction
Asexual Reproduction
The Chromosomal Basis of Sex Determination in Plants

KARYOTYPE ANALYSIS
Introduction
Nomenclature of Chromosomes
Karyotype Analysis by Mitotic Metaphase Chromosomes
Karyotype Analysis by Pachytene Chromosomes
Karyotype Analysis by Flow Cytometry
Karyotype Analysis by Image Analysis
Chromosome Number of Economically Important Plants

CHROMOSOMAL ABERRATIONS - STRUCTURAL CHROMOSOME CHANGES
Deficiencies
Duplications
Interchanges
Inversions

CHROMOSOMAL ABERRATIONS - NUMERICAL CHROMOSOME CHANGES
Euploidy
Aneuploidy: Trisomics
Monosomics and Nullisomics
Alien Addition Lines
Alien Substitution Lines

GENOME ANALYSIS
Introduction
Classical Taxonomy and Genome Designations
Genome Relationships among Diploid Species
Genomes of Glycine
Genomes of Gossypium
Ancestors of Allopolyploid Species
Genome Analysis by Molecular Methods

CHROMOSOMAL ABERRATIONS IN CELL AND TISSUE CULTURE
Introduction
Chromosomal Aberrations in Callus
Mechanism of Chromosomal Aberrations in Culture
Chromosomal Aberrations in Regenerated Plants
Chromosomal Aberrations in Haploid Callus and Their Regenerants
Chromosomal Aberrations in Somatic Hybrids

TRANSGENIC CROPS
Introduction
Present Status of Transgenic Crops
Production of Transgenic Crops
Chromosome Aberrations in Transgenic Crops
Cytological Basis of Gene Silencing

APPENDICES

REFERENCES

GLOSSARY

O autorze

Ram J. Singh