ISBN-13: 9781071615577 / Angielski / Twarda / 2021 / 390 str.
ISBN-13: 9781071615577 / Angielski / Twarda / 2021 / 390 str.
“A unique feature is the inclusion of step-by-step protocols to replicate or add these techniques to ongoing research.” (Whitney A Welch, Doody's Book Reviews, December 3, 2021)
Part I: Reviews
1. The New Frontier of Three-Dimensional Culture Models to Scale Up Cancer Research
Caleb Jensen, Chloe Shay, and Yong Teng
2. Therapeutic Effects of Resveratrol on Non-Alcoholic Fatty Liver Disease through Inflammatory, Oxidative Stress, Metabolic, and Epigenetic Modifications
Mina Karimi, Behnaz Abiri, Paul C. Guest, and Mohammadreza Vafa
3. A Matter of Fat: Body Fat Distribution and Cardiometabolic Disease in Africa
Nasrin Goolam Mahyoodeen and Nigel J. Crowther
4. PGK1: An Essential Player in Modulating Tumor Metabolism
Leslie Duncan, Chloe Shay, and Yong Teng
5. The Association of Reproductive Aging with Cognitive Function in Sub-Saharan African Women
Nicole G. Jaff and Nigel J. Crowther
6. The Effects of Exercise on Lipid Biomarkers
Michael F. Mendoza, Sergey M. Kachur, and Carl J. Lavie
7. Exercise Training Protocols to Improve Obesity, Glucose Homeostasis, and Subclinical Inflammation
Tiego A. Diniz, Barbara M. Antunes, Jonathan P. Little, Fabio S. Lira, and José Cesar Rosa-Neto
Part II: Protocols
8. A Protocol of Intradialytic Exercise for Improvements in Inflammatory Status, Body Composition, and Functional Capacity
Lorena Cristina Curado Lopes, Paula Alves Monteiro, and João Felipe Mota
9. Evaluating the Anticancer Activity of Natural Products Using a Novel 3D Culture Model
Chloe Shay and Yong Teng
10. Evaluation of Antidiabetic Properties of Adenosma bracteosum Bonati Extracts in Mice with Streptozotocin-Induced Diabetes
Giau Van Vo, Paul C. Guest, and Ngoc Hong Nguyen
11. Testing the Effects of Cinnamon Extract Supplementation on Inflammation and Oxidative Stress Induced by Acrylamide
Fatemeh Haidari, Majid Mohammadshahi, Behnaz Abiri, Paul C. Guest, Mehdi Zarei, and Mojdeh Fathi
12. Proteomic Mapping of the Human Myelin Proteome
Paul C. Guest
13. Testing for Thyroid Peroxidase and Anti-Neuronal Antibodies in Depression and Schizophrenia
Johann Steiner, Winfried Stoecker, Bianca Teegen, Henrik Dobrowolny, Gabriela Meyer-Lotz, Katrin Borucki, Paul C. Guest, and Hans-Gert Bernstein
14. Evaluation of Antimicrobial and Anticancer Activities of Bouea macrophylla Ethanol Extract
Giau Van Vo, Paul C. Guest, and Ngoc Hong Nguyen
15. Evaluation of Anti-Hepatocellular-Cancer Properties of β-Sitosterol and β-Sitosterol-Glucoside from Indigofera zollingeriana Miq
Giau Van Vo, Paul C. Guest, Thuy Trang Nguyen, and Tuong Kha Vo
16. Label-Free Electrochemical Biosensors to Evaluate the Antioxidant Effect of Tocopherol in Ultraviolet Radiation
Lixia Gao and Yong Teng
17. qRT-PCR Analysis of GLUT-4 and Assessment of Trolox as an Effective Antioxidant in Diabetic Cardiomyoblasts
S. Asha Devi, Ravichandra Shivalingappa Davargaon, and Subramanyam Muthangi VV
18. Flow Cytometric Analysis of Hyperglycemia-Induced Cell Death Pathways in Cardiomyoblasts
Ravichandra Shivalingappa Davargaon, Subramanyam Muthangi VV, and S. Asha Devi
19. Vietnamese Medicinal Plants as Potential Resources to Explore New Anti-Cancer and Anti-Inflammation: Established Assays for Pharmacological Tests
Trang Thi Phuong Nguyen, Dieu Thi Xuan Nguyen, and Triet Thanh Nguyen
20. Testing the Effect of Curcumin on Proliferative Capacity of Colorectal Cancer Cells
Tannaz Jamialahmadi, Paul C. Guest, Amir R. Afshari, Muhammed Majeed, and Amirhossein Sahebkar
21. Assessment of Topical and Transdermal Penetration of Curcuma heyneana Rhizome Extract in Rat Skin: Histological Analysis
Idha Kusumawati, Rohmania, Mega Ferdina Warsito, and Eka Pramyrtha Hestianah
22. Measuring the Effects of Berberine on Serum Pro-Oxidant–Antioxidant Balance in Metabolic Syndrome
Tannaz Jamialahmadi, Paul C. Guest, Aida Tasbandi, Khalid Al-Rasadi, and Amirhossein Sahebkar
23. Testing the Anti-Inflammatory Effects of Curcuminoids in Patients with Colorectal Cancer
Tannaz Jamialahmadi, Paul C. Guest, Aida Tasbandi, Muhammed Majeed, and Amirhossein Sahebkar
24. Analytical Methods and Bioassays for Cytotoxicity and Anti-Diabetic Properties of Aquilaria Crassna Leaf Extracts in HepG2 Cells
Pinnara Rojvirat, Netiya Karaket, Phanupol Mongkolsiri, and Sarawut Jitrapakdee
25. Testing the Physical and Molecular Effects of Nutritional Supplements and Resistance Exercise in Middle-Aged Females
Behnaz Abiri, Paul C. Guest, Parvin Sarbakhsh, and Mohammadreza Vafa
26. Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells
Mohammad Jalili-Nik, Amir R. Afshari, Khadijeh Mahboobnia, Paul C. Guest, Tannaz Jamialahmadi, and Amirhossein Sahebkar
27. Protocol for Testing the Potential Antioxidant Effects of Curcuminoids on Patients with Type 2 Diabetes Mellitus
Tannaz Jamialahmadi, Yunes Panahi, Muhammed Majeed, Paul C. Guest, and Amirhossein Sahebkar
28. The Detection of Toxic Compounds in Extracts of Callilepis laureola (Ox-Eye Daisy) and Senecio latifolius (Ragwort) by Ultra-Performance Liquid Chromatography Mass Spectrometry (UPLC-MS/MS)
Tracy Snyman and Nigel J. Crowther
29. Impact of Curcumin on Hepatic Low-Density Lipoprotein Uptake
Mohammad Jalili-Nik, Khadijeh Mahboobnia, Paul C. Guest, Muhammed Majeed, Khalid Al-Rasadi, Tannaz Jamialahmadi, and Amirhossein Sahebkar
This detailed book explores protocols with the aim of testing aerobic exercise, resistance training, special diets, additives and natural products, which have led to new insights into the physiological and molecular aspects of health and disease. Many of these approaches have contributed to significant improvements in disease areas such as cardiovascular disease, cognitive dysfunction, diabetes, frailty, gliobastoma, metabolic syndrome, obesity, oxidative stress, and various cancers. This collection also provides important information on disease mechanisms and novel drug targets as each protocol is presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
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