Pharmacological Assays of Plant-Based Natural Products

ISBN-13: 9783319268101 / Angielski / Twarda / 2016 / 188 str.

Parimelazhagan Thangaraj

Opis

This volume provides information on how to select and screen plants for their medicinal properties. It describes phytopharmacological techniques for extracting and qualitatively and quantitatively analyzing a plant's phytochemicals. After a detailed in vitro investigation including nutritional and anti-nutritional analyses, medicinal properties were tested with various in vivo models for anti-inflammatory, analgesic, anti-pyretic, anticancer and anti-diabetic properties, as well as wound healing, neurodegenerative diseases, etc. Compound identification and purification techniques include, among others, TLC and column chromatography, as well as molecular docking with specific proteins.

Spis treści

1. Extraction of plant material.- 2. Critical aspects of purification and impurities in natural product extracts. 3. In vitro antioxidant assays.- 4. In vivo antioxidant assays.- 5. Quantification assays.- 6. Anti-nutritional factors.- 7. Anti-inflammatory activity.- 8. Antipyretic activity.- 9. Inflammation bowel disease.- 10. Anti-arthritic activity.- 11. Evaluation of analgesic potential.- 12. Anti-diarrhoeal activity.- 13. Anti-ulcer activity.- 14. In vitro anti-diabetic activity.- 15. In vivo anti-diabetic activity.- 16. Hepatoprotective property.- 17. Antitumor activity.- 18. In vivo wound healing studies.- 19. Determination of cytotoxicity.- 20. Evaluation of bioavailability.- 21. Molecular identification by DNA barcoding.- 22. Molecular docking of bioactive compounds against BRCA and COX proteins.

 

 

 

 

O autorze

Parimelazhagan Thangaraj is a Professor in the Department of Botany, Bharathiar University, Coimbatore – 641 046, India. His field of specialization is Bioprospecting of Medicinal Plants. He is currently engaged in compilation and documentation on available knowledge on valuable plant resources to prove their utility scientifically through detailed phytochemical, biological and pharmacological investigations.