Phage Display: Methods and Protocols

ISBN-13: 9781493974467 / Angielski / Twarda / 2017 / 562 str.

Michael Hust; Theam Soon Lim

Opis

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display. This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Construction of Antibody Phage Display Libraries

1. Construction of Human Immune and Naïve scFv Libraries

Jonas Kügler, Florian Tomszak, André Frenzel, and Michael Hust

2. Construction of Naïve and Immune Human Fab Phage Display Library

Noorsharmimi Omar and Theam Soon Lim

3. Construction of Synthetic Antibody Phage Display Libraries

Johan Nilvebrant and Sachdev S. Sidhu

4. Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes

Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, and Bin Liu

5. Construction of Macaque Immune Libraries

Arnaud Avril, Sebastian Miethe, Michael Hust, and Thibaut Pelat

6. Construction o

f Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) Format

Ulrike S. Diesterbeck

7. Construction of Rabbit Immune Antibody Libraries

Thi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim

8. Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries

Julius Grzeschik, Doreen Könning, Simon Krah, Christian Schröter, Martin Empting, Harald Kolmar, and Stefan Zielonka

9. Construction of High-Quality Camel Immune Antibody Libraries

Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, and Baptiste Monsion

10. Construction of Chicken Antibody Libraries

Jeanni Fehrsen, Susan Wemmer, and Wouter van Wyngaardt

11. Construction and Selection of Affilin Phage Display Libraries

Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, and Eva

Bosse-Doenecke

12. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies

Déborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau

13. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach

Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung

Part II Selection Strategies for Antibodies

14. Parallelized Antibody Selection in Microtiter Plates

Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel, and Michael Hust

15. Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning

Chai Fung Chin, Yee Siew Choong, and Theam Son Lim

16. Magnetic Nanoparticle-Based Semi-Automated Panning for

High-Throughput Antibody Selection

Angela Chiew Wen Ch’ng, Nurul Hamizah binti Hamidon, Zoltán Konthur, and Thean Soon Lim

17. Phage Display and Selections on Cells

Wieland Fahr and André Frenzel

18. Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens

Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, and Bin Liu

19. Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies

Almog Bitton, Limor Nahary, and Itai Benhar

20. Screening Phage Display Antibody Libraries using Protein arrays

Ricardo Jara-Acevedo, Paula Díez, María González-González, Rosa María Dégano, Nieves Ibarrola, Rafael Góngora, Alberto Orfao, and Manuel Fuentes

21. Antibody

Selection on FFPE Tissue Slides

Andre ten Haaf, Stefan Gattenlöhner, and Mehmet Kemal Tur

22. Antibody Affinity and Stability Maturation by Error Prone PCR

Tobias Unkauf, Michael Hust, and André Frenzel

Part III Complementary Approaches for Antibody Phage Display Selections

23. Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data 

Christian Grohmann and Michael Blank

24. Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction

Kevin A. Henry

25. High-Throughput IgG Reformatting and Expressions

Chao-Guang Chen, Georgina Sansome. Michael J. Wilson, and Con Panousis

26. Monitoring Phage Biopanning by Next-Generation Sequencing

Anna Vaisman-Mentesh and Yariv Wine

Part IV Phage Display for Epitope Mapping and Identification of Biomarkers

27. ORFeome Phage Display

Jonas Zantow, Gustavo Marçal Schmidt Garcia Moreira, Stefan Dübel, and Michael Hust

28. Epitope Mapping by Phage Display

Gustavo Marçal Schmidt Garcia Moreira, Viola Fühner, Michael Hust

29. Metasecretome Phage Display

Milica Ciric, Filomena Ng, Jasna Rakonjac, and Dragana Gagic

30. Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages

Gertrudis Rojas and Tania Carmenate