Peptide and Protein Engineering: From Concepts to Biotechnological Applications

ISBN-13: 9781071607220 / Angielski / Miękka / 2021 / 289 str.

Olga Iranzo; Ana Cecília Roque

Spis treści

1. SEA Ligation is Accelerated at Midly Acidic pH: Application to the Formation of Difficult Peptide Junctions

            Marine Cargoët, Vincent Diemer, Laurent Raibaut, Elizabeth Lissy, Benoît Snella, Vangelis Agouridas, and Oleg Melnyk

 

2. The Problem of Aspartimide Formation during Protein Chemical Synthesis Using SEA-Mediated Ligation

            Jennifer Bouchenna, Magalie Sénéchal, Hervé Drobecq, Jérôme Vicogne, and Oleg Melnyk

 

3. Using the Interactive Tool of the Protein Chemical Synthesis Database

            Vangelis Agouridas and Oleg Melnyk

 

4. Only a “Click” Away: Development of Arginine-Rich Peptide-Based Materials Using Click Chemistry

            Mariana Barbosa, Fabíola Costa, Cátia Teixeira, M. Cristina L. Martins, and Paula Gomes

 

5. Fluoroglycoproteins by Copper-Free Strain-Promoted Azide–Alkyne Cycloaddition

            Pedro M.S.D. Cal, Gonçalo J.L. Bernardes, and Omar Boutureira

 

6. Hybrid Silylated Peptides for the Design of Bio-Functionalized Materials

            Titouan Montheil, Cecile Echalier, Jean Martinez, Ahmad Mehdi, and Gilles Subra

 

7. Synthesis of Peptide-Oligoethylene Glycol (OEG) Conjugates for Multivalent Modification of Nanomaterials

            Daniel Pulido and Miriam Royo

 

8. Phage Display Methodologies

            Agathe Urvoas, Philippe Minard, and Patrice Soumillion

 

9. Whole-Bacterium Ribosome Display Selection for Isolation of Anti-Bacterial Affitins

            Ghislaine Béhar, Stanimir Kambarev, Jennifer Jazat, Barbara Mouratou, and Frédéric Pecorari

 

10. CIS Display: DNA-Based Technology as a Platform for Discovery of Therapeutic Biologics

            Ana Margarida Gonçalves Carvalho Dias

 

11. Building Scarless Gene Libraries in the Chromosome of Bacteria

            Gol Mohammad Dorrazehi, Sebastian Worms, Jason Baby Chirakadavil, Johann Mignolet, Pascal Hols, and Patrice Soumillion

 

12. Streptavidin (Sav)-Based Artificial Metalloenzymes: Cofactor Design Considerations and Large Scale Expression of Host Protein Variants

            Juliane Klehr, Jingming Zhao, Amanda Santos Kron, Thomas R. Ward, and Valentin Köhler

 

13. Integrated Bacterial Production of Large Combinatorial Libraries of Cyclic Oligopeptides and Functional Screening for the Identification of Chemical Rescuers of Pathogenic Protein Misfolding and Aggregation

            Dafni C. Delivoria and Georgios Skretas

 

14. Site-Specific Incorporation of Non-Canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli

            Uchralbayar Tugel, Meritxell Galindo, and Birgit Wiltschi

Blurb

This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice. 

Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering.