Peptide Nucleic Acids: Methods and Protocols

ISBN-13: 9781493959525 / Angielski / Miękka / 2016 / 239 str.

Peter E. Nielsen; Daniel H. Appella

Opis

This volume details methods and protocols in PNA chemistry. It features step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Spis treści

1. MiniPEG-gPNA

Arunava Manna, Srinivas Rapireddy, Raman Bahal, and Danith H. Ly

 

2. Cyclopentane Peptide Nucleic Acids

Ethan A. Englund, Ning Zhang, Daniel H. Appella

 

3. Chiral PNAs with Constrained Open-chain Backbones

Roberto Corradini, Tullia Tedeschi, Stefano Sforza and Rosangela Marchelli

 

4. Synthesis, Characterization and Evaluation of Radiometal-Containing Peptide Nucleic Acids

Holger Stephan, Christian Förster, and Gilles Gasser

 

5. Preparation of Metal-Containing Peptide Nucleic Acid Bioconjugates on the Solid Phase

Gilles Gasser

 

6. Formation and Characterization of PNA-Containing Heteroquadruplexes

Bruce A. Armitage

 

7. Sequence Selective Recognition of Double Stranded RNA using Triple Helix-forming Peptide Nucleic Acids

Thomas Zengeya, Pankaj Gupta, and Eriks Rozners

 

8. Assembly of PNA-tagged Small Molecules, Peptides and Carbohydrates onto DNA Templates: Programming the  Combinatorial Pairing and Inter-ligand Distance

Nicolas Winssinger, Katarzyna Gorska, Mihai Ciobanu , Jean Pierre Daguer, and Sofia Barluenga

 

9. Site-selective Scission of Human Genome using PNA-based Artificial Restriction DNA Cutter

Kenichiro Ito and Makoto Komiyama

 

10. PNA Openers and their Applications for Bacterial DNA Diagnostics

Irina V. Smolina  and Maxim D. Frank-Kamenetskii

 11. DNA-templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids – A Versatile Tool for Single Base Specific Detection of Nucleic Acids

Alexander Roloff, Simon Ficht, Christian Dose, and Oliver Seitz

 

12. Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods

Stefano Sforza, Tullia Tedeschi, Mariangela Bencivenni, Alessandro Tonelli, Roberto Corradini, and Rosangela Marchelli

 

13. Analysis of PNA Hybridization by Surface Plasmon Resonance

Bruce A. Armitage

 14. PNA Fluorescent in situ Hybridization (FISH) For Rapid Microbiology and Cytogenetic AnalysisHenrik Stender, Brett William, and James Coull

 

15. Rapid miRNA Imaging in Cells using Fluorogenic Templated Staudinger Reaction Between PNA-based Probes

Katarzyna Gorska and Nicolas Winssinger

 

16. Cellular Delivery of Peptide Nucleic Acids (PNAs)

Takehiko Shiraishi and Peter E. Nielsen

 

17. Peptide Nucleic Acid Mediated Recombination For Targeted Genomic Repair and Modification

Erica B. Schleifman and Peter M. Glazer

 

18. Antisense Effects of PNAs in Bacteria

Shan Goh, Jem Stach, and Liam Good

 

Blurb

Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Peptide Nucleic Acids: Methods and Protocols, Second Edition serves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.