Peptide Characterization and Application Protocols

ISBN-13: 9781617376375 / Angielski / Miękka / 2010 / 342 str.

Gregg B. Fields

Opis

This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.

Spis treści

Peptide Characterization and Application Protocols Preface Contributors Section 1: Characterization Chapter 1: HPLC Analysis and Purification of Peptides Colin T. Mant, Yuxin Chen, Zhe Yan, Traian V. Popa, James M. Kovacs, Janine B. Mills, Brian P. Tripet, and Robert S. Hodges Chapter 2: Identification of Proteins Based on MS/MS Spectra and Location of Post-translational Modifications Kathryn L. Stone, Myron Crawford, Walter McMurray, Nancy Williams, and Kenneth R. Williams Chapter 3: Determination of Phosphorylated and O-glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature Gary M. Hathaway Chapter 4: Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein-Coupled Receptor Fred Naider Section 2: Application Chapter 5: Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors Janelle L. Lauer-Fields, Dmitriy Minond, Keith Brew, and Gregg B. Fields Chapter 6: Proteolytic Profiling of the Extracellular Matrix Degradome Diane Baronas-Lowell, Janelle L. Lauer-Fields, Mohammad Al-Ghoul, and Gregg B. Fields Chapter 7: Š-Amyloid Protein Aggregation Inhibition Marcus A. Etienne, Nadia J. Edwin, Jed P. Aucoin, Paul S. Russo, Robin L. McCarley, and Robert P. Hammer Chaper 8: Radiometal-labeled Somatostatin Analogues for Applications in Cancer Imaging and Therapy Jason S. Lewis and Carolyn J. Anderson Chapter 9: Cell Penetrating Proline Rich Peptidomimetics Josep Farrera-Sinfreu, Ernest Giralt, Miriam Royo, and Fernando Albericio Chapter 10: Peptide-Mediated Targeting of Liposomes to Tumor Cells Evonne M. Rezler, David R. Khan, Raymond Tu, Matthew Tirrell, and Gregg B. Fields Chapter 11: Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells Sébastien Deshayes, Federica Simeoni, May C. Morris, Gilles Divita, and FrédéricHeitz Chaper 12: Broth Microdilution Antibacterial Assay of Peptides Laszlo Otvos and Mare Cudic Chapter 13: Depsipeptide Synthesis Maciej Stawikowski and Predrag Cudic

Blurb

Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.