1. Identification of PDZ Interactions by Yeast Two-Hybrid Monica Castro-Cruz, Marta Monserrat-Gomez, Jean-Paul Borg, Pascale Zimmermann, and Eric Bailly
2. Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis Avais M. Daulat , Stéphane Audebert, Mônica Wagner, Luc Camoin, and Jean-Paul Borg
3. Identification of PDZ Interactions by Proteomic Peptide Phage Display Susanne Lüchow, Gustav Sundell, and Ylva Ivarsson
4. A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay Pau Jané, Lionel Chiron, Goran Bich, Gilles Travé, and Yves Nominé
5. Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore Pascale Zimmermann and Antonio Luis Egea-Jimenez
6. PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations Célia Caillet-Saguy, Sébastien Brûlé, Nicolas Wolff, and Bertrand Raynal
7. Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module Janesha C. Maddumage, Bryce Z. Stewart, Patrick O. Humbert, and Marc Kvansakul
8. A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics Young Joo Sun and Ernesto J. Fuentes
9. Unveiling the Folding Mechanism of PDZ Domains Candice Gautier and Stefano Gianni
10. Development of Peptide-Based PDZ Domain Inhibitors Dominik J. Essig, Javier R. Balboa, and Kristian Strømgaard
11. Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs Márton A. Simon and László Nyitray
12. Chemical Synthesis of PDZ Domains Christin Kossmann, Sana Ma, Louise S. Clemmensen, and Kristian Strømgaard
13. Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains Carlos Castaño-Rodriguez, Jose M. Honrubia, Javier Gutiérrez-Álvarez, Isabel Sola, and Luis Enjuanes
14. Computational Design of PDZ:peptide Binding Nicolas Panel, Francesco Villa, Vaitea Opuu, David Mignon, and Thomas Simonson
15. Mechanoregulation of PDZ Proteins, An Emerging Function Elsa Bazellières and André Le Bivic
16. Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains Laurent Hoffer, Philippe Roche, and Xavier Morelli
Subject Index List…
This volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, PDZ Mediated Interactions: Methods and Protocols is a valuable resource for all researchers interested in learning more about this developing field.