PCR Primer Design

ISBN-13: 9781617377433 / Angielski / Miękka / 2010 / 432 str.

Anton Yuryev

Opis

At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

Spis treści

PCR primer design. Contents Part I. Basic Principles and software for PCR primer design Chapter 1. Physical Principles and Visual-OMP Software for Optimal PCR Design Chapter 2. Oligo 7 Primer Analysis Software Chapter 3. Selection for 3' end triplets for polymerase chain reaction primers Chapter 4. The reference point method in primer design Chapter 5. PCR primer design using statistical modeling Chapter 6. Developing a Statistical Model for Primer Design Part II. Genome scale PCR primer design Chapter 7. GST-PRIME: An Algorithm for Genome-Wide Primer Design Chapter 8. Genome-Scale Probe and Primer Design with PRIMEGENS Repeat masking for PCR primer design Chapter 9. SNPbox: web-based high-throughput primer design with an eye for repetitive sequences. Chapter 10. Fast masking of repeated primer binding sites in eukaryotic genomes Multiplex PCR primer design Chapter 11. Degenerate Primer Design: Theoretical Analysis and the HYDEN program Chapter 12. An Iterative Method for Selecting Degenerate Multiplex PCR Primers Chapter 13. Primer-design for multiplexed genotyping Chapter 14. MultiPLX: automatic grouping and evaluation of PCR primers Chapter 15. MultiPrimer: A system for Microarray PCR Primer Design Allele-specific PCR Chapter 16. Modified oligonucleotides as tools for allele-specific amplification Chapter 17. AlleleID: A Pathogen Detection and Identification System Long PCR primer design Chapter 18. GenoFrag, a software package for the design of primers dedicated to whole genome scanning by LR-PCR. DNA methylation mapping Chapter 19. Designing PCR primer for DNA methylation mapping Chapter 20. BiSearch: ePCR tool for native or bisulfite treated genomic template. Chapter 21. Graphical design of primers with PerlPrimer

Blurb

In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome.

In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists – geneticists, molecular biologists, molecular geneticists, and more – who routinely use PCR in their research.