P53 Protocols

ISBN-13: 9781493959334 / Angielski / Miękka / 2016 / 238 str.

Sumitra Deb; Swati Palit Deb; Swati Palit Deb

Opis

In its updated 2nd edition, this Methods in Molecular Biology (TM) book offers tools for identifying target genes, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, generation of pluripotent stem cells and more.

Spis treści

1. Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues

Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon

 

2. Detecting p53 Isoforms at Protein Level

Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon

 

3. Autophagy, Senescence and Apoptosis

Rachel W. Goehe, Molly L. Bristol, Eden N. Wilson, and David A. Gewirtz

 

4. p53 and Cell Cycle Effects after DNA Damage

Emir Senturk, James J. Manfredi

 

5. p53 Ubiquitination and Proteasomal Degradation

Ian Love, Dingding Shi, Steven R. Grossman

 

6. Identification of p53 in Mitochondria

Angelina V. Vaseva and Ute M. Moll

 

7. Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis

Sumitra Deb and Paul R. Graves

 

8. Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization

Qi Zhang, and Hua Lu

 

9. Determine the Effect of p53 on Chemosensitivity

Emir Senturk, James J. Manfredi

 

10. Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells

Mahesh Ramamoorthy, Catherine Vaughan, Sumitra Deb, Swati Palit Deb

 

11. Mutant p53 in Cell Adhesion and Motility

W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb

12. Use of the DNA Fiber Spreading Technique to Detect the  Effects of Mutant p53 on DNA Replication

Rebecca A. Frum, Sumitra Deb and Swati Palit Deb

 

13. Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts

Evguenia M. Alexandrova and Ute M. Moll

 

14. p53 Actions on MicroRNA Expression and Maturation Pathway

Hiroshi I. Suzuki and Kohei Miyazono

 

15. Isolation and Characterization of Murine Multipotent Lung Stem Cells

Venkat S Gadepalli, Catherine A Vaughan, Raj R. Rao

 

16. Generation of p53 Knock-down Cell Lines

Catherine Vaughan, Swati Palit Deb, Sumitra Deb

 

17. ChIP for Identification of p53 Responsive DNA Promoters

Jun-Ming Liao  and Hua Lu

 

18. ChIP-on-chip to Identify Mutant p53 Targets

Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino

 

19. ChIP Sequencing to Identify p53 Targets

Catherine Vaughan, Brad Windle, Sumitra Deb

 

20. The p53-Mdm2 Loop: A Critical Juncture of Stress Response

Yaara Levav-Cohen, Zehavit Goldberg, Valentina Zuckerman, Sue Haupt, and Ygal Haupt

Blurb

The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field.  These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.