Oxidative Stress and Nanotechnology: Methods and Protocols

ISBN-13: 9781627034746 / Angielski / Twarda / 2013 / 294 str.

Donald Armstrong; Dhruba J. Bharali

Opis

The emergence of nanotechnology has had a profound effect on almost every aspect of the 21st century's daily life. It has had a revolutionary impact from stain-resistant clothing and cosmetics to environmental issues, including energy and medicine and even aerospace engineering. In Oxidative Stress and Nanotechnology: Methods and Protocols, expert researchers in the field detail various aspects of nanotechnology from the oxidative stress point of view. Focusing on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action along with their clinical relevance. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oxidative Stress and Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of techniques for nanotechnology in pathophysiology and protection from the oxidative stress point of view.

Spis treści

Part I: Reviews

1. Nanoscale Biosensor for Detection of Reactive Oxygen Species

Tarl W. Prow, Daniel Sundh, and Gerard A. Lutty

 

2. Nanoparticles: Toxicity, Radicals, Electron Transfer and Antioxidants

Peter Kovacic and Ratnasamy Somanathan

 

3. Encapsulation of Anti-Oxidants in Gastrointestinal Resistant Nanoparticulate Carriers

Eliana B. Souto, Patrícia Severino, Rafael Basso, and Maria Helena A. Santana

 

4. Toxicity of Novel Nanosized Formulations Used in Medicine

Afaf El-Ansary , Sooad Al-Daihan, Abir Ben Bacha , and Malak Kotb

 

5. Fullerenol Nanoparticles: Toxicity and Antioxidant Activity

Rade Injac, Matevz Prijatelj, and Borut Strukelj

 

6. Hydrogen Peroxide (H₂O₂) Detection with Nanoprobes for Biological Applications: A Mini-Review

Gwangseong Kim, Yong-Eun Koo Lee, and Raoul Kopelman

 

7. Studying the Oxidative Stress Paradigm in vitro: A theoretical and Practical Perspective

Martin J. D. Clift and Barbara Rothen-Rutishauser

 

8. Evaluation of the Effect of Acute and Sub-acute Exposure to TiO₂ Nanoparticles on Oxidative Stress

Shichang LIU, Zhuo YANG

 

9. Biomedical Applications of Nano-antioxidant

Geeta Watal, Aparna Watal , Prashant Kumar Rai, Devendra Kumar Rai, Bechan Sharma

 

Part II: Protocols

10. Lipid Peroxidation Due to In Vitro and In Vivo Exposure of Biological Samples to Nanoparticles

Anca Dinischiotu, Loredana Stanca,  Daniela Gradinaru, Sorina Nicoleta Petrache,  Mihaela Radu, Andreea Iren Serban

 

11. Testing Nanomaterial Toxicity in Unicellular Eukaryotic Algae and Fish Cell Lines

Alexandra Kroll, Dana Kühnel, and Kristin Schirmer

 

12. Gold Nanoparticles and Oxidative Stress in the Blue Mussel, Mytilus edulis

Sara Tedesco, Hugh Doyle, Daniela Iacopino, Irene O’Donovan, Sarah Keane, and David Sheehan

 

13. Oxidative Biomarkers to Assess the Nanoparticles Induced Oxidative Stress

A. Rama Narsimha Reddy, Y. Narsimha Reddy, and Krishna R. Devarakonda

 

14. Vesicular Antioxidants: Role in Age-related Cerebral Oxidative Injury

Sibani Sarkar, Ardhendu Kumar Mandal, and Nirmalendu Das

  

15. Methods for Detection of Oxidative Stress and Genotoxicity of Engineered Nanoparticles

Ashutosh Kumar, Vyom Sharma, and Alok Dhawan

 

16. Size Dependent Study of Pulmonary Responses to Nano-sized Iron and Copper Oxide Nanoparticles

Rajiv Kumar and Dattatri K Nagesha

 

17. In vitro Techniques to Investigate the Oxidative Effects of Quantum Dots

Ridhima Juneja and Indrajit Roy

 

18. Hybrid Polymeric Nanoparticles: Potential Candidate for Ophthalmic Delivery

Dhruba J Bharali, Donald Armstrong, and Shaker A Mousa

 

Part III: Future Directions

19. Relevance of Nanotechnology in Modulating Oxidative Stress:  An Overview

Dhruba J Bharali and Shaker A Mousa

 

Blurb

The emergence of nanotechnology has had a profound effect on almost every aspect of the 21st century’s daily life. It has had a revolutionary impact from stain-resistant clothing and cosmetics to environmental issues , including energy and medicine and even aerospace engineering.  In Oxidative Stress and Nanotechnology: Methods and Protocols, expert researchers in the field detail various aspects of nanotechnology from the oxidative stress point of view. Focusing on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action along with their clinical relevance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Oxidative Stress and Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of techniques for nanotechnology in pathophysiology and protection from the oxidative stress point of view.