Organelle Proteomics

ISBN-13: 9781617377723 / Angielski / Miękka / 2010 / 421 str.

Delphine Pflieger; Jean Rossier

Opis

This is the first book to examine organelle proteomics in depth.

It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis. Detailed protocols are provided and a section is devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle.

Spis treści

1. - Analysis of organelles by on-line two-dimensional liquid chromatography – tandem mass spectrometry (2DLC-MS/MS) Edwin P. Romijn and John R. Yates III. Analysis of organelles, vesicles and protein machineries 2 - Purification and proteomic analysis of chloroplasts and their sub-organellar compartments Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro 3 – Proteomic analysis of the stacked Golgi complex Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu 4 - Purification of Saccharomyces cerevisiae mitochondria by zone-electrophoresis in a free flow device (ZE-FFE) Hans Zischka, Norbert Kinkl, Ralf J. Braun and Marius Ueffing 5 - Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild Type and Mutant Mitochondria: Activity Measurement and Subunit Composition Analysis Claire Lemaire and Geneviève Dujardin 6 - Mitochondrial proteomics: Analysis of a whole mitochondrial extract with two-dimensional electrophoresis Thierry Rabilloud. 7 - Purification and proteomic analysis of the mouse liver mitochondrial inner membrane Sandrine Da Cruz and Jean-Claude Martinou 8 - Subcellular Fractionation and Proteomics of Nuclear Envelopes Laurence Florens, Nadia Korfali and Eric C. Schirmer. 9 - Purification and proteomic analysis of the nuclear insoluble protein fraction Tsuneyoshi Horigome, Kazuhiro Furukawa and Kohei Ishii 10 - Preparation Methods of Human Metaphase Chromosomes for Their Proteome Analysis. Kiichi Fukui and Hideaki Takata 11 - Purification and proteomic analysis of plant plasma membranes Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom and Christer Larsson 12 - Protocol to enrich and analyze plasma membrane proteins from frozen tissue Jacek R. Wisniewski 13 - Proteomic characterization of membrane proteintopology Adele R. Blackler and Christine C. Wu 14 - Free Flow Isoelectric Focussing (FF-IEF): A method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes Markus Islinger and Gerhard Weber 15 - Use of gas-phase-fractionation to increase protein identifications: application to the peroxisome Jacob Kennedy and Eugene C. Yi 16 - Purification and proteomic analysis of lysosomal integral membrane proteins Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran, John W. Callahan. 17 - Affinity purification of soluble lysosomal proteins for mass spectrometric identification Sylvie Kieffer-Jaquinod, Agnès Chapel, Jérôme Garin and Agnès Journet 18 - Purification and proteomic analysis of synaptic vesicles Holly D. Cox and Charles M. Thompson. 19 – Purification and proteomic analysis of pancreatic zymogen granule membranes Xuequn Chen and Philip C. Andrews 20 - Isolation and proteomic analysis of Chlamydomonas centrioles Lani C. Keller and Wallace F. Marshall. 21 - Purification and proteomic analysis of 20S proteasomes from human cells Marie-Pierre Bousquet-Dubouch, Sandrine Uttenweiler-Joseph, Manuelle Ducoux-Petit, Mariette Matondo, Bernard Monsarrat, and Odile Burlet-Schiltz. 22 – Characterization of E. coli ribosomal particles: combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography - tandem mass spectrometry. Isabelle Iost, Julie Charollais, Joëlle Vinh and Delphine Pflieger Proteins identified in the organelle sample: true components or contaminants? 23 - Assessment of organelle purity using antibodies and specific assays: the example of the chloroplast envelope Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro 24 - Identifying bona fide components of an organelle by isotope coded labelling of

Blurb

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.