Next-Generation Microrna Expression Profiling Technology: Methods and Protocols

ISBN-13: 9781617794261 / Angielski / Twarda / 2011 / 341 str.

Jian-Bing Fan

Opis

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

Spis treści

1. Controlling miRNA Regulation in Disease

            Willemijn M. Gommans and Eugene Berezikov

 

2. Introduction to miRNA Profiling Technologies and Cross-Platform Comparison

            Sarah Aldridge and James Hadfield

 

3. Stem-Loop RT-qPCR for MicroRNA Expression Profiling

            James Hurley, Doug Roberts, Andrew Bond, David Keys, and Caifu Chen

 

4. Poly(T) Adaptor RT-PCR

            Rui Shi, Ying-Husan Sun, Xing-Hai Zhang, and Vincent L. Chiang

 

5. MicroRNA In Situ Hybridization

            Boye Schnack Nielsen

 

6. Agilent MicroRNA Microarray Profiling System

            Petula N. D’Andrade and Stephanie Fulmer-Smentek

 

7. miRNA Gene Expression Profiling Using Illumina Universal BeadChips

            Jing Chen, Craig S. April, and Jian-Bing Fan

 

8. MicroRNA Expression Analysis Using the Affymetrix Platform

            Suzanne Dee and Robert C. Getts

 

9. Individualized miRNA Assay Panels Using Optically Encoded Beads

            Keld Sorensen

 

10. Microfluidic Primer Extension Assay (MPEA)

            Markus Beier and Valesca Boisguérin

 

11. MicroRNA Profiling Using µParaflo Microfluidic Array Technology

            Xiaochuan Zhou, Qi Zhu, Christoph Eicken, Nijing Sheng, Xiaolin Zhang, Litao Yang, and Xiaolian Gao

 

12. MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform

            Shujun Luo

 

13. Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application

            Ana Raquel Soares, Patrícia M. Pereira, and Manuel A.S. Santos

 

14. Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing

            Sam E.V. Linsen and Edwin Cuppen

 

15. Profiling of Short RNAs Using Helicos Single-Molecule Sequencing

            Philipp Kapranov, Fatih Ozsolak, and Patrice M. Milos

 

16. deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data

            Jian-Hua Yang and Liang-Hu Qu

 

17. PhenomiR: MicroRNAs in Human Diseases and Biological Processes

            Andreas Ruepp, Andreas Kowarsch, and Fabian Theis

 

18. miRNA Expression Profiling: From Reference Genes to Global Mean Normalization

            Barbara D’haene, Pieter Mestdagh, Jan Hellemans, and Jo Vandesompele

 

19. miRNA Data Analysis: Next-Gen Sequencing

            Preethi H. Gunaratne, Cristian Coarfa, Benjamin Soibam, and Arpit Tandon

 

20. Integrated miRNA Expression Analysis and Target Prediction

            William Ritchie and John E.J. Rasko

 

21. miRNAs in Human Cancer

            Xiaomin Zhong, George Coukos, and Lin Zhang

 

22. Blood-Based miRNA Preparation for Non-Invasive Biomarker Development

            Svenja Debey-Pascher, Jing Chen, Thorsten Voss, and Andrea Staratschek-Jox