New Antibiotic Targets

ISBN-13: 9781617378430 / Angielski / Miękka / 2010 / 274 str.

W. Scott Champney

Opis

A crisis is developing in the treatment of infectious diseases, with microorganisms frequently showing resistance to currently prescribed antibiotics. Fewer and fewer antimicrobial agents are now available to treat these infections. In New Antibiotic Targets, W. Scott Champney examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies. New Antibiotic Targets provides laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases."

Recenzje

From the reviews:

"This book describes, in specific detail, a variety of methods used to identify cellular components of bacteria that would be good targets for antibiotics. ... Those working in laboratories on discovery of new antibiotics are the intended audience. The book is easy to follow and written in a step-wise fashion. It would be easy for laboratory technicians as well as graduate students to follow. ... This is a good methods book that contains the latest information on new techniques." (Rebecca T. Horvat, Doody's Review Service, May, 2008)

"The contributors to each chapter are good and well known names in their fields, providing good coverage of their subjects. I think this book will find utility in the field of drug discovery and target identification specifically from compound libraries ... ." (Paul Hoskisson, Microbiology Today, July, 2008)

Spis treści

New Antibiotic Targets Book Chapter Titles 1. Biocomputational Strategies for Microbial Drug Target Identification 2. Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities 3. A Method to Assay Inhibitors of DNA Polymerase III Activity 4. Methods to Identify and Characterize Inhibitors of Bacterial RNA Polymerase 5. Methods to Assay Inhibitors of tRNA Synthetase Activity 6. Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly 7. Inhibition of Chaperone-dependent Bacterial Ribosome Biogenesis 8. Assays for the Identification of Inhibitors Targeting Specific Translational Steps 9. SPARK - A New Peptidyl Transferase Activity Assay 10. High-throughput Screening of Peptide Deformylase Inhibitors 11. A Method to Assay Penicillin Binding Proteins 12. A Method to Assay Inhibitors of Lipopolysaccharide Synthesis 13. Methods for Assessing the Structure and Function of Cationic Antimicrobial Peptides 14. Flow Cytometry of Bacterial Membrane Potential and Permeability 15. Bacterial Efflux Pump Inhibitors 16. Mycobacterium tuberculosis Beta-ketoacyl Acyl Carrier Protein Synthase III (mtFabH) Assay - Principle and Method 17. Screening for Compounds that Affect the Interaction between Bacterial Two- Component Signal Transduction Response Regulator Protein and Cognate Promoter DNA 18. The Activity of rRNA Resistance Methyltransferases Assessed by MALDI Mass Spectrometry 19. Assays for Beta-lactamase Activity and Inhibition 20. Studies of Enzymes that Cause Resistance to Aminoglycoside Antibiotics

Blurb

A crisis is developing in the treatment of infectious diseases, with microorganisms frequently showing resistance to currently prescribed antibiotics. Fewer and fewer antimicrobial agents are now available to treat these infections. In "New Antibiotic Targets," W. Scott Champney examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies.

With detailed methods and protocols for the identification and assay of these targets, "New Antibiotic Targets" provides laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases.