Neurodegeneration Methods and Protocols

ISBN-13: 9780896036123 / Angielski / Twarda / 1998 / 306 str.

Jean Harry; Hugh A. Tilson

Opis

Rapid advances in our understanding of basic cell biological processes and of the molecular mechanisms of cell function and dysfunction have led to an increasing interest in utilizing these approaches in neurobiological research. Efforts in the most rapidly advancing areas are multidisciplinary and the o- come of contributions from many investigators employing a variety of te- niques to address a specific problem. Although a strong basis in fundamental neurobiological concepts is essential for each researcher, the ability to apply new techniques and approaches to the examinination of both cellular and molecular processes requires knowledge of a wide variety of methodologies. The objective of Neurodegeneration Methods and Protocols is to develop an understanding, appreciation, and technical ability in various cellular and molecular techniques for studying many aspects of nervous system cell bi- ogy. The protocols in this volume span a multidisciplinary range of cellular and molecular approaches, and should allow investigators to address research questions directed toward understanding nervous system function, injury, degeneration, and the repair/regenerative process.

Recenzje

". . .the authors are to be commended for the consistency in format and altruism shown contributors to represent the procedures with minimal self-serving recounting of their use of the method."-The Quarterly Review of Biology

Spis treści

Introduction to Polymerase Chain Reaction-PCR, Mark C. Miller and Laura Cunningham. Methodological Issues in Polymerase Chain Reaction for RNA, Gregory S. Kelner and Richard A. Maki. Subtractive Hybridization of cDNA Libraries, Gregory S. Kelner and Richard A. Maki. The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in Ischemic Brain Injury, Zinkang Wang and Giora Z. Feurestein. Analysis of Gene Expression By Multiprobe RNase Protection Assay, Anna K. Stalder, Axel Pagenstecher, Carrie L. Kincaid, and Ian L. Campbell. Molecular Probes for PNS, Degeneration, and Regeneration,Arrel D. Toews. Mobility Shift DNA-Binding Assay Using Gel Electrophoresis,Keith Pennypacker and Yang Xiao. Transfection of Mammalian Cells inVitro: Use in Analysis of Neuronal Damage, Melvin L. Billingsley and Theresa A. Thompson. Detection of Apoptotic Cells in the Nervous System, Terri Kagan and Zahra Zakeri. Translocation Assays of Protein Kinase C Activation, David M. Terrian, Ginger G. Wescott, and Christina M. Manring. Astrocyte and Neuron Coculturing Method, Michael Aschner and Barbara A. Bennett. Viability Assays for Cells in Vitro: The Ethidium/Calcein Assay and the Immunofluorescense Combination Assay, Theresa A. Thompson. Measurement of Nitric Oxide Synthase Activity Using the Citrulline Assay, Thomas R. Ward and William R. Mundy. Measurement of Calcium Buffering by Intracellular Organelles in Brain, Prasada Rao S. Kodavanti. Measurement of Intraneuronal Free Calcium Using Fluorescent Probe Technique, Prasada Rao S. Kodavanti. Receptor-Mediated Release of Inositolphosphates in Brain Slices, Pushpa Tandon. The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins, Dori Germolec. Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders, Eliezer Masliah and Michael Alford. Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue, Paul E. Gottschall, J. Wenjun Zhang, and Suman Deb. The Measurement of MonoamineNeurotransmitters in Microdialysis Perfusates Using HPLC-ECD, Ian Acworth and Michael L. Cunningham. Biochemical Measurement of Cholinesterase Activity, Stephanie Padilla, T. Leon Lassiter, and Deborah Hunter. Functional Study of Glutamate Receptor Channels in Brain Slices, Susan Jones and Jerrel L. Yakel. Selective Silver Staining for Neural Degeneration, Karl F. Jensen and Jeanene K. Olin. Immunohistological Markers of Cerebrovascular Integrity,Karl F. Jensen, Jeanene K. Olin, Julie A. Varner, and Robert L. Isaacson. Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells, Richard M. LoPachin and Christopher L. Gaughan.

Blurb

In Neurodegeneration Methods and Protocols, Jean Harry and Hugh Tilson bring together a collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. The methods span a multidisciplinary range of cellular and molecular approaches to both normal and injured nervous system function, and particularly to neurodegenerative processes. Included are basic RT-PCR techniques, cell culture systems, second-messenger signaling methods, and patch-clamp techniques.
Cutting-edge and authoritative, Neurodegeneration Methods and Protocols will illuminate nervous system function, injury, degeneration, and the repair/regenerative process. These powerful techniques will also allow established laboratories-both experimental and clinical-to expand their array of methods for solving research problems and help utilize multidisciplinary procedures beyond their experience.