Nanoproteomics: Methods and Protocols

ISBN-13: 9781493957996 / Angielski / Miękka / 2016 / 322 str.

Steven A. Toms; Robert J. Weil

Opis

This volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research.

Spis treści

Part I: Preliminary Sample Preparation

 

1. MALDI-TOF Serum Profiling Using Semi-Automated Serum Peptide Capture with Magnetic Reversed Phase (C18) Beads

            Jaco C. Knol and Connie R. Jimenez

 

2. Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling

            Lorenza Franciosi, Natalia Govorukhina, Nick ten Hacken, Dirkje Postma, and Rainer Bischoff

 

Part II: Nanoscale Separation

 

3. Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces

            Rui Vitorino, Jana Krenkova, Frantisek Foret, Pedro Domingues, and Francisco Amado

 

4. Three Dimensional Peptide Fractionation for Highly Sensitive Nanoscale LC-Based Shotgun Proteomic Analysis of Complex Protein Mixtures

            Sricharan Bandhakavi, Todd W. Markowski, Hongwei Xie, and Timothy J. Griffin

 

5. Nanospray Ion Mobility Mass Spectrometry of Selected High Mass Species

            Iain Campuzano and Kevin Giles

 

6. Nanoelectrospray-MSⁿ of Native and Permethylated Glycans

            Christina Bleckmann, Hildegard Geyer, and Rudolf Geyer

 

7. N-linked Global Glycan Profiling by NanoLC Mass Spectrometry

            Michael S. Bereman and David C. Muddiman

 

8. Applications of Nanoscale Liquid Chromatography Coupled to Tandem Mass Spectrometry in Quantitative Studies of Protein Expression, Protein-Protein Interaction, and Protein Phosphorylation

            Metodi V. Metodiev

 

9. Nano LC-MS/MS: A Robust Setup for Proteomic Analysis

            Marco Gaspari and Giovanni Cuda

 

10. Nanofluidic Devices for Rapid Continuous-Flow Bioseparation

            Pan Mao and Jianping Fu

 

11. Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring (SRM)

            Thomas Fröhlich and Georg J. Arnold

 

Part III: Nanomaterials: Nanostructured Surfaces

 

12. A Sample Preparation Method for Gold Nanoparticle-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

            Yen-Hsiu Lin and Wei-Lung Tseng

 

13. Nanostructured TiO₂ Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry

            Federico Torta, Matteo Fusi, Carlo S.Casari, Andrea Li Bassi, and Angela Bachi

 

14. Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry

            Chia-Wen Tsao and Don L. DeVoe

 

15. Protein Nanoarrays for High Resolution Patterning of Bacteria on Gold Surfaces

            Cait Costello, Jan-Ulrich Kreft, Christopher M. Thomas, and Paula M. Mendes

 

Part IV: Nanomaterials: Nanoscale Tools for Proteomic Discovery

 

16. Engineered Multifunctional Nanotools for Biological Applications

            Mohammed Ibrahim Shukoor, Muhammad Nawaz Tahir, Thomas Schladt, Wolfgang Tremel, Zhiqun Zhang, Kevin K. Wang, and Firas Kobeissy

 

17. The Selective Capture of Phosphopeptides by Zirconium Phosphonate-Modified Magnetic Nanoparticles

            Liang Zhao, Ren’an Wu, and Hanfa Zou

 

18. Nanowire Biosensors for Label-Free, Real-Time, Ultrasensitive Protein Detection

            Gengfeng Zheng and Charles M. Lieber

 

19. Generation of Anti-Infectome/Anti-Proteome Nanobodies

            Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, and Serge Muyldermans

 

Part V: Nanotechnology: Techniques in Proteomics--Potential Application for Health & Disease

 

20. Isolation, Propagation, and Analysis of Biological Nanoparticles

            Michael P. Linnes, Farooq A. Shiekh, Larry W. Hunter, Virginia M. Miller, and John C. Lieske

 

21. Functionalized Soluble Nanopolymers for Phosphoproteome Analysis

            Anton Iliuk, Keerthi Jayasundera, Rachel Schluttenhofer, and W. Andy Tao

 

22. Elucidating Structural Dynamics of Integral Membrane Proteins on Native Cell Surface by Hydroxyl Radical Footprinting and NanoLC-MS/MS

            Yi Zhu, Tiannan Guo, and Siu Kwan Sze

 

23. Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC) to the Characterization of Proteome, Glycoproteome, and Phosphoproteome Using Nano LC-MS/MS

            Piliang Hao, Huoming Zhang, and Siu Kwan Sze

Blurb

As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues.  The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular Biology™, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls.

 

Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.