Nadph Oxidases: Methods and Protocols

ISBN-13: 9781493994267 / Angielski / Miękka / 2020 / 718 str.

Ulla G. Knaus;Thomas L. Leto

Opis

This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational "from bed to bench and back" studies. Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on NADPH oxidase regulation and their function in cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, NADPH Oxidases: Methods and Protocols will aid researchers working with the NOX/DUOX family in continuing and expanding upon their vital research.

Spis treści

Part I: The NADPH Oxidase Family: Overviews

 

1. Intersecting Stories of the Phagocyte NADPH Oxidase and Chronic Granulomatous Disease

            William M. Nauseef and Robert A. Clark

 

2. Mammalian NADPH Oxidases

            Hélène Buvelot, Vincent Jaquet, and Karl-Heinz Krause

 

Part II: Biochemistry and Model Systems

 

3. Enhanced Immunoaffinity Purification of Human Neutrophil Flavocytochrome B for Structure Determination by Electron Microscopy

            Algirdas J. Jesaitis, Marcia Riesselman, Ross Taylor, and Susan Brumfield

 

4. Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs)

            Jennifer L. Meitzler

 

5. A Close-Up View of the Impact of Arachidonic Acid on the Phagocyte NADPH Oxidase

            Tania Bizouarn, Hager Souabni, Xavier Serfaty, Aicha Bouraoui, Rawand Masoud, Gilda Karimi, Chantal Houée-Levin, and Laura Baciou

 

6. NOX5 Cell-Free Assay for the High-Throughput Screening of Small Molecules

            Fiona Augsburger, Delphine Rasti, Yves Cambet, and Vincent Jaquet

 

7. Spectroscopy of NOX Protein Family Members

            Yoko Nakano and William M. Nauseef

 

8. Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases

            Hideki Sumimoto, Reiko Minakami, and Kei Miyano

 

9. Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays

            Mary C. Dinauer

 

10. The X-CGD PLB-985 Cell Model for NOX2 Structure-Function Analysis

            Sylvain Beaumel and Marie José Stasia

 

11. Functional Characterization of DUOX Enzymes in Reconstituted Cell Models

            Agnieszka Korzeniowska, Ágnes P. Donkó, Stanislas Morand, and Thomas L. Leto

 

12. Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR

            Becky A. Diebold, S. Garrett Wilder, Xavier De Deken, Jennifer L. Meitzler, James H. Doroshow, James W. McCoy, Yerun Zhu, and J. David Lambeth

 

Part III: Detection of ROS

 

13. Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells

            Fiona Augsburger, Aleksandra Filippova, and Vincent Jaquet

 

14. HPLC-Based Monitoring of Oxidation of Hydroethidine for Detection of NADPH Oxidase-Derived Superoxide Radical Anion

            Jacek Zielonka, Monika Zielonka, and Balaraman Kalyanaraman

 

15. Visualization of Intracellular Hydrogen Peroxide with the Genetically-Encoded Fluorescent Probe HyPer in NIH-3T3 Cells

            Yulia G. Ermakova, Nataliya M. Mishina, Carsten Schultz, and Vsevolod V. Belousov

 

16. Imaging Intracellular H₂O₂ with the Genetically-Encoded PerFRET and OxyFRET Probes

            Balázs Enyedi and Miklós Geiszt

 

17. Quantitative Imaging of Endogenous and Exogenous H₂O₂ Gradients in Live Zebrafish Larvae

            Mark Jelcic, Balázs Enyedi, and Philipp Niethammer

 

18. Kinetic Analysis of Phagosomal ROS Generation

            Sophie Dupré-Crochet, Marie Erard, and Oliver Nüβe

 

19. Imaging Intestinal ROS in Homeostatic Conditions Using L-012

            Emer Conroy and Gabriella Aviello

 

20. Hydro-Cy3-Mediated Detection of Reactive Oxygen Species In Vitro and In Vivo

            Bejan J. Saeedi, Bindu Chandrasekharan, and Andrew S. Neish

 

Part IV: Regulation of NADPH Oxidase

 

21. Phosphorylation of Gp91^phox/NOX2 in Human Neutrophils

            Houssam Raad, Riad Arabi Derkawi, Asma Tlili, Sahra A. Belambri, Pham My-Chan Dang, and Jamel El-Benna

 

22. The Molecular Regulation and Functional Roles of NOX5

            David J.R. Fulton

 

23. Using Synthetic Peptides for Exploring Protein-Protein Interactions in the Assembly of the NADPH Oxidase Complex

            Edgar Pick

 

24. Rational Design and Delivery of NOX-Inhibitory Peptides

            Eugenia Cifuentes-Pagano and Patrick J. Pagano

 

25. High-Throughput Screening of NOX Inhibitors

            Jacek Zielonka, Monika Zielonka, Gang Cheng, Micael Hardy, and Balaraman Kalyanaraman

 

26. Protein-Protein Interaction Assay to Analyze NOX4/p22^phox Heterodimerization

            Sharon O’Neill and Ulla G. Knaus

 

Part V: Function of NADPH Oxidase

 

27. Isolation of Redox Active Endosomes (Redoxosomes) and Assessment of NOX Activity

            Weam S. Shahin and John F. Engelhardt

 

28. Model Systems to Investigate NOX-Dependent Cell Migration and Invasiveness

            Howard E. Boudreau and Thomas L. Leto

 

29. NADPH Oxidases and Aging Models of Lung Fibrosis

            Karen Bernard and Victor J. Thannickal

 

30. Proteomic Methods to Evaluate NOX-Mediated Redox Signaling

            Christopher M. Dustin, Milena Hristova, Caspar Schiffers, and Albert van der Vliet

 

31. Neutrophil Extracellular Traps

            Balázs Rada

 

Part VI: Genetic Disorders

 

32. Chronic Granulomatous Disease

            Dirk Roos

 

33. Diagnostic Testing for Chronic Granulomatous Disease

            Douglas B. Kuhns

 

34. Gastrointestinal Complications in Chronic Granulomatous Disease

            E. Liana Falcone and Steven M. Holland

 

35. Ex Vivo Models of Chronic Granulomatous Disease

            Julie Brault, Bénédicte Vigne, and Marie Jose Stasia

 

36. Gene Editing in Chronic Granulomatous Disease

            Colin L. Sweeney, Randall K. Merling, Suk See De Ravin, Uimook Choi, and Harry L. Malech

 

37. DUOX Defects and Their Roles in Congenital Hypothyroidism

            Xavier De Deken and Françoise Miot

 

38. NAPDH Oxidases in Inflammatory Bowel Disease

            Emily Stenke, Billy Bourke, and Ulla G. Knaus