Multiplexed Imaging: Methods and Protocols

ISBN-13: 9781071615928 / Angielski / Twarda / 2021 / 343 str.

Eli Zamir

Spis treści

1.     Strategies for Multiplexed Biosensor Imaging to Study Intracellular Signaling Networks

Jeremiah Keyes, Sohum Mehta, and Jin Zhang

 

2.     Six-color Confocal Immunofluorescence Microscopy with 4-Laser Lines

Lukas Heger, Jennifer J. Lühr, Lukas Amon, Ana-Sunčana Smith, Nathalie Eissing, and Diana Dudziak

 

3.     Multiplexed Imaging of Posttranslational Modifications of Endogenous Proteins in Live Cells

Yuko Sato and Hiroshi Kimura

 

4.     Multiplex Imaging of Rho GTPase Activities in Living Cells

Ravi M. Bhalla, Maren Hülsemann, Polina V. Verkhusha, Myla G. Walker, Daria Shcherbakova, and Louis Hodgson

 

5.     Multicolor Localization-Based Super Resolution Microscopy

Leila Nahidiazar and Rolf Harkes

 

6.     Multiplexed Tissue Tomography

Evan H. Phillips, David Scholten, Amy C. Flor, Stephen J. Kron, and Steve Seung-Young Lee

 

7.     Multicolor 3D Confocal Imaging of Thick Tissue Sections

Leo Kunz and Daniel L. Coutu

 

8.     Multiphoton Deep-Tissue Imaging of Micrometastases and Disseminated Cancer Cells Using Conjugates of Quantum Dots and Single-Domain Antibodies

Alyona Sukhanova, Fernanda Ramos-Gomes, Patrick Chames, Pavel Sokolov, Daniel Baty, Frauke Alves, and Igor Nabiev

 

9.     Multiplexed Imaging for immune profiling on Human FFPE Material

Artur Mezheyeuski and Carina Strell

 

10.  Method for Multiplexed Dynamic Intravital Multi-Photon Imaging

Asylkhan Rakhymzhan, Andreas Acs, Ruth Leben, Thomas H. Winkler, Anja E. Hauser, and Raluca A. Niesner

 

11.  Fourier Multiplexed Fluorescence Lifetime Imaging

Leilei Peng

 

12.  Bimolecular Fluorescence Complementation (BiFC) and Multiplexed Imaging of Protein-Protein Interactions in Human Living Cells

Yunlong Jia, Françoise Bleicher, Jonathan Reboulet, and Samir Merabet

 

13.  Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging under Adverse Optical Conditions

Raja Chouket, Ruikang Zhang, Agnès Pellissier-Tanon, Annie Lemarchand, Agathe Espagne, Thomas Le Saux, and Ludovic Jullien

 

14.  Multicolor Bioluminescence Imaging of Subcellular Structures and Multicolor Calcium Imaging in Single Living Cells

Kazushi Suzuki, Md Nadim Hossain, Tomoki Matsuda, and Takeharu Nagai

 

15.  Nanoparticles for In Vivo Lifetime Multiplexed Imaging

Erving Ximendes, Emma Martín Rodríguez, Dirk Ortgies, Meiling Tan, Guanying Chen, and Blanca del Rosal

 

16.  Versatile on-Demand Fluorescent Labeling of Fusion Proteins using Fluorescence-Activating and Absorption-Shifting Tag (FAST) 

Arnaud Gautier, Ludovic Jullien, Chenge Li, Marie-Aude Plamont, Alison G. Tebo, Marion Thauvin, Michel Volovitch, and Sophie Vriz

 

17.  UltraPlex Hapten-Based Multiplexed Fluorescent Immunohistochemistry

Matt Levin, Amy Flor, Helen Snyder, Stephen J. Kron, and David Schwartz

 

18.  Multi-Modal Approach for Cancer Cells Investigation

Alexandre Berquand and Jerôme Devy

 

19.  Multiplexed Fourier Transform Infrared and Raman Imaging

Guillermo Quintás, Bayden R. Wood, Hugh J. Byrne, and David Perez-Guaita

 

20.  Multiplexed Imaging Mass Spectrometry of Histological Staining, N-glycan and Extracellular Matrix from One Tissue Section: A Tool for Fibrosis Research

Cassandra L. Clift, Anand Mehta, Richard R. Drake, and Peggi M. Angel

 

21.  Multiplexed Raman Imaging in Tissues and Living Organisms

Travis M Shaffer and Sanjiv S. Gambhir

Blurb

This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.