Multiple Myeloma: Methods and Protocols

ISBN-13: 9781617375378 / Angielski / Miękka / 2010 / 310 str.

Ross D. Brown; P. Joy Ho

Opis

A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets."

Recenzje

From the reviews:

"...extremely informative...a great reference for researchers or students with an interest in the etiology and treatment of Multiple Myeloma." - The Journal of the Association of Genetic Technologists

"Audience: Hematologists, oncologists, and other clinicians ... as well as biomedical researchers in the field of hematology and immunology. ... The book is divided into 23 chapters, including schematic diagrams, figures, color plates, and tables, as well as an index of important terms at the end. ... covers clinical as well as experimental aspects of multiple myeloma and provides an important set of information for clinicians and researchers, in different fields of biomedicine, including hematology, oncology, molecular diagnostic, clinical immunology, osteoimmunology, molecular-biology, genetics, and bioinformatics." (Danka Grcevic, Croatian Medical Journal, Vol. 48, October, 2007)

Spis treści

Multiple Myeloma: Challenges and Opportunities Douglas Joshua, Ross D. Brown, and P. Joy Ho Immunophenotyping of Plasma Cells in Multiple Myeloma Gema Mateo Manzanera, Jesús F. San Miguel Izquierdo, and Alberto Orfao de Matos Plasma Cell Labeling Index Philip R. Greipp and Shaji Kumar Conventional Cytogenetics in Myeloma Lynda J. Campbell Multicolor Spectral Karyotyping in Multiple Myeloma Jeffrey R. Sawyer Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization Scott VanWier and Rafael Fonseca Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma Pulivarthi H. Rao Southern Blotting of IgH Rearrangements in B-Cell Disorders Edna Yuen and Ross D. Brown Identification and Assembly of V Genes As Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma Surinder S. Sahota, Mark Townsend, and Freda K. Stevenson Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma Brian J. Taylor, Jitra Kriangkum, Erin R. Strachan, Juanita Wizniak, and Linda M. Pilarski Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma Mara Compagno, Barbara Mantoan, Monica Astolfi, Mario Boccadoro, and Marco Ladetto Incomplete DJH Rearrangements David González and Ramón Garcia-Sanz Identification of Malignant Plasma Cells by mRNA In Situ Hybridization Ross D. Brown and P. Joy Ho The SCID-hu Myeloma Model Joshua Epstein and Shmuel Yaccoby The 5T2MM Murine Model of Multiple Myeloma: Maintenance and Analysis Karin Vanderkerken, Kewal Asosingh, Angelo Willems, Hendrik De Raeve, Pedro Couck, Frans Gorus, Peter Croucher, and Ben Van Camp Determination of Telomerase Activity and Telomere Length Kai-da Wu andMalcolm A. S. Moore Dendritic Cell/Myeloma Hybrid Vaccine Dajing Xia, Tim Chan, and Jim Xiang Genetically Engineered Myeloma Cell Vaccine Siguo Hao, Tim Chan, and Jim Xiang An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors Andrew C. W. Zannettino, Amanda N. Farrugia, L. Bik To, and Gerald J. Atkins Clonality Detection of the Expanded T-Cell Populations in Patients With Multiple Myeloma Daniel M-Y. Sze Detecting Mismatch Repair Defects in Myeloma Mark Velangi, Elizabeth Matheson, Andrew Hall, and Julie Irving Methylation-Specific Polymerase Chain Reaction Oliver Galm and James G. Herman Making Sense of DNA Microarray Data Wei-min Liu, Sunhee K. Ro, and Walter H. Koch Index

Blurb

Although there is no therapy that could be considered curative for patients with multiple myeloma, recent research has gained considerable momentum and yielded a real prospect for achieving a significant improvement in response to therapy. In Multiple Myeloma: Methods and Protocols, established researchers with hands-on experience detail a comprehensive set of classic and innovative methodologies used in many laboratories for the investigation of this disease. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology, which is used routinely in many laboratories around the world to determine prognosis, to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Multiple Myeloma: Methods and Protocols offers both novice and experienced researchers alike a gold-standard collection of proven techniques for investigating the pathogenesis of multiple myeloma and identifying new therapeutic targets.