Monoterpene contents in Mentha arvensis L.

ISBN-13: 9786200529923 / Angielski / Miękka / 2020 / 56 str.

Muhammad Akram; Faheem Aftab

Opis

We present a comparative study of monoterpenes in in vitro and field grown plants of Mentha arvensis L. (Japanese mint). Apical and nodal explants were cultured on MS agar-solidified media + BAP (0.2 to 1 mgL-1) for shoot induction. Highest shoot induction was obtained at 1mgL-1 BAP from both explants. The number of shoots was highest at 0.75 mgL-1 Kinetin and 1mgL-1 BAP from axenic shoots. In vitro grown shoots were cultured in MS salts + IBA (2 mgL-1) for rooting. Excellent callus was achieved from leaf explants cultured on MS medium + 2,4-D 3 mgL-1 or 0.25 mgL-1. In vitro, field grown and callus were subjected for extraction and evaluation of monoterpenes. There were 17 constituents detected from field grown plants by Gas Chromatography- Mass Spectrometery (GC-MS) of which menthol was the major component (57.44%) as compared to menthone (9.17%) and pulegone (7.73%), whereas, menthone with 65.32% was the main component of oil extracted from 16 weeks old calli followed by menthol (0.11%) and pulegone (0.21%). Mint oil extracted from in vitro plants showed variable concentration of 15 different compounds .

O autorze

Akram, Muhammad
Dr. Muhammad Akram is a teacher and researcher of Plant Tissue Culture working as Assistant Professor of Botany at Govt. Postgraduate College Sahiwal, Pakistan. He has vast experience of disease-free potato seed production and in vitro multiplication of trees and medicinal plants.