Molecular investigations on archival tissues

ISBN-13: 9783639168471 / Angielski / Miękka / 2009 / 104 str.

Jorunn Litlekalsoy

Opis

Nowadays there are many available molecular methods for studying diseases. Archival tissue provides an extremely valuable material for proper understanding of pathology of various diseases. A major problem in molecular analysis of archival tissues is the tissue degradation that might occur during long storage. This monograph presents various methods for efficient extraction of DNA and protocols for sequencing (detection of gene mutations) from archival tissues. It also provides protocols for immunodetection of various proteins on such archival tissues collected at Gades Institute, Haukeland University Hospital, Norway in the period 1932 till 1996. In addition this book illustrates the value of studies on archival tissues for detecting changes in the pathogenesis of diseases with time. In this monograph bladder cancer is used as an example for such studies. This book could be useful for professionals, considering the use of archival tissue to study mechanisms of various diseases.

O autorze

Jorunn Litlekalsoy is educated as engineer in analytical
chemistry at Norwegian High School of Engineering. She
obtained an MPhil in Molecular Biology at University of Bergen
in 2005 on establishing methods for molecular analysis of
archival tissue stored at Gades Institute. She is continuing
this work in a PhD study at Gades Institute.