Molecular and Modern Studies on Potato leaf roll virus (PLRV)

ISBN-13: 9783659553448 / Angielski / Miękka / 2014 / 152 str.

Mohamed Dwidar Emad Fathy

Opis

This investigation was carried out to achieve the following: 1. Conducting some molecular studies on PLRV such as : • Extraction of RNA • Reverse transcriptiom reaction • PCR • Purification of PCR product • Cloning of PCR product • Large scale plasmid preparation(Maxi-prep) • Subcloning • Large scale plasmid preparation(Maxi-prep) 2. Using of modern serological methods for rapid detection of PLRV such as: • ELISA • DBIA • TBIA 3. Recognize the intracellular changes induced by the PLRV using Electron Microscopy 4. Production of PLRV-free potato plants.

O autorze

Name: Prof.Dr. Emad Fathy DwidarDate of Birth: May 27, 1971Position: Professor of Viruses, Botany Department, Faculty of Agriculture, Fayoum University, EgyptResearch Interests: Molecular Virology, Molecular Biology, Diagnosis of Viral Diseases, Inhibition Of Viral Infection, Production of virus-free plants using Tissue Culture Techniques.