Molecular Pathology Protocols

ISBN-13: 9780896036819 / Angielski / Twarda / 2000 / 491 str.

Anthony A. Killeen

Opis

The era of molecular pathology has arrived. From its promising beg- nings in research laboratories, the field has grown, and continues to grow, to become a vital part of the care of an ever-increasing number of patients. Because of its recent emergence from the research taboratory, many molecuIar pathology protocols we still to be found in the primary litcramre, and have not appeared in a text. MO PCU Q Padhoiogy Protocob contains la- ratory protocoIs that have been developed by many of the authors for use in clinical molecular pathology laboratories and describe in detail Row to perform these assays. This book is therefore intended for clinical laboratory use by medical technologists and pathologists. It will dso be of interest to research workers who are performing these assays. In its broadest meaning, pathology is the study of disease, and therefore it follows that any disease for which the molecular basis is understood would be suitable as a topic for inclusion in this work. When seiecting protocols, it was necessary to place limits on the number of chapters that could be feasibly presented in a single work. Those protoculs that were selected are performed more frequently, or have achieved recognition as having important diagnostic utility in contemporary practice. A decision was made to exclude inherited genetic diseases with certain exceptions, such as those diseases that are associated with thrombotic states and are part of the traditional dumain of pathology.

Recenzje

"It contains cutting-edge molecular diagnostic techniques with detailed step-by-step instructions and tips to obtain successful results....the purpose here is to offer readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. It is a worthy objective and the editor meets it very well....Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology....Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays....The significant diagnostic utility of molecular protocols in cancer, hermatological malignancies, and virus related diseases is covered well....It is a good protocol resource book in molecular pathology."-Doody's Health Sciences Book Review Journal

"Molecular Pathology Protocols fills a unique niche for general molecular pathology laboratories that may be called upon to perform a wide variety of tests for infectious and noninfectious diseases. It has 35 chapters that provide extensive detail on laboratory protocols for a variety of important conditions, from techniques for preparation of nucleic acid templates and mutation detection for genetic and neoplastic evaluations, to hematological applications, to a limited number of detailed protocols for infectious disease diagnosis and monitoring...The major strength of the protocols discussed in this book lies in their enormous detail and the practical remarks of the authors, which will assist in establishing the assays in a new laboratory. Another great value in some chapters is the emphasis on the assessment of quality control that will meet or exceed all the requirements of agencies that license and regulate clinical laboratories." - Clinical Infectious Diseases

"A must for genetic laboratories and all those using the new molecular biological methods." - Journal of Endocrine Genetics

"Each protocol in this book includes sections of introduction, materials, methods, and notes. Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology. Most protocols use polymerase chain reaction (PCR) based techniques. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in sity hybridization assays. This book has good references in both background of diseases and techniques. Most charts and labels have high quality;...The significant diagnostic utility of molecular protocols in cancer, hematological malignancies, and virus related diseases is covered well....This book is well written and the molecular protocols introduced can be easily followed practically." - E-Streams

Spis treści

DNA Extraction from Paraffin-Embedded Tissues Hongxin Fan and Margaret L. Gulley DNA Extraction from Fresh or Frozen Tissues Hongxin Fan and Margaret L. Gulley RNA Extraction from Fresh or Frozen Tissues Hongxin Fan and Margaret L. Gulley Single-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53 Gene Kirsi H. Vähäkangas, Katariina Castrén, and Judith A. Welsh Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection Laura Heisler and Chao-Hung Lee Detection of Telomerase by In Situ Hybridization and by the Polymerase Chain Reaction-Based Telomerase Activity Assay Carmela P. Morales and Shawn E. Holt Detection of Microsatellite Instability Karin D. Berg, Constance A. Griffin, and James R. Eshleman Polymerase Chain Reaction Clonality Assays Based on X-Linked Genes Langxing Pan and Huaizheng Peng Fluorescent In Situ Hybridization: Evaluation for Ploidy and Gene Amplification Susan Sheldon HER-2/neu Oncogene Amplification Determined by Fluorescence In Situ Hybridization, Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl Linda M. Wasserman Detection of t(15;17)(q24;q21), inv(16)/t(16;16)(p13;q22), and t(8;21)(q22;q22) Anomalies in Acute Myeloid Leukemia David S. Viswanatha Detection of t(14;18)(q32;q21)-Associated BCL-2/JH Gene Fusion in Non-Hodgkin's Lymphoma David S. Viswanatha Detection of Breast Cancer Cells Using Immunomagnetic Beads and Reverse Transcription-Polymerase Chain Reaction Scott Luke and Karen L. Kaul Molecular Detection of Circulating Prostate Cancer Cells Karen L. Kaul Methods to Detect Clonal Gene Rearrangements in Lymphomas and Leukemias Naheed Mitha and Ronald C. McGlennen Monitoring of Bone Marrow TransplantEngraftment Kristine P. Woronzoff-Dashkoff and Ronald C. McGlennen Direct Molecular Diagnosis of Multiple Endocrine Neoplasia Type 1 Elizabeth M. Petty, Michael Glynn, and Allen E. Bale Molecular Detection of Multiple Endocrine Neoplasia Type 2 Edward H. Rowsell and Myra J. Wick Assay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis Coli Gene Stephen B. Gruber Detection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ Hybridization Elizabeth R. Unger and Suzanne D. Vernon Molecular Methods for Detecting Epstein-Barr Virus (Part I): Hybridization to Epstein-Barr Virus-Encoded RNA (EBER) Transcripts Hongxin Fan and Margaret L. Gulley Molecular Methods for Detecting Epstein-Barr Virus (Part II): Structural Analysis of Epstein-Barr Virus DNA as a Marker of Clonality Hongxin Fan and Margaret L. Gulley Molecular Methods for Detecting Epstein-Barr Virus (Part III): EBV Viral Load by Competitive Polymerase Chain Reaction Hongxin Fan and Margaret L. Gulley Molecular Detection of Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus-8 Ethel Cesarman Diagnostic Applications of Quantitative Polymerase Chain Reaction for Cytomegalovirus, Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2 Yi-Wei Tang Detection and Typing of Hepatitis C Virus Frederick S. Nolte Detection and Speciation of Mycobacteria in Formalin-Fixed, Paraffin-Embedded Tissue Sections Diana Mohl and Thomas J. Giordano Ultrasensitive Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by the AMPLICOR and COBAS AMPLICOR HIV-1 MONITORTM Tests Steven Herman, James Novotny, and Maurice Rosenstraus Molecular Diagnosis of Hereditary Thrombotic Disorders James G. Donnell

Blurb

Because molecular pathology has only recently emerged from the laboratory, many molecular pathology protocols are still to be found only in the primary literature. In Molecular Pathology Protocols, Anthony Killeen has assembled a collection of readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. Each method is described in step-by-step detail by a leading molecular pathologist or laboratory scientist who has developed it or used it extensively. These clinical laboratory techniques can be used for the diagnosis or monitoring of cancer, hematological malignancies, infectious diseases, and selected genetic disorders. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays. Time-tested practices and numerous tips on how to avoid pitfalls ensure robust and successful results.
Comprehensive and path-breaking, Molecular Pathology Protocols will enable clinical laboratories to introduce new molecular pathology tests and lay the groundwork for a much-needed standardization in this rapidly developing field.