Molecular Microbial Diagnostic Methods: Pathways to Implementation for the Food and Water Industries

ISBN-13: 9780124169999 / Angielski / Twarda / 2015 / 262 str.

Cook, Nigel D'Agostino, Martin Thompson, K. Clive

Opis

Molecular Microbial Diagnostic Methods: Pathways to Implementation for the Food and Water Industry was developed by recognized and experienced highlevel scientists. It's a comprehensive and detailed reference that uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. This book discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The content is also pertinent for anyone carrying out microbiological analysis at the research level, and for scientists developing methods, as it focuses on the requirements of end-users.

• Includes information on how to introduce and implement molecular methods for routine monitoring in food and water laboratories
• Discusses the importance of robust validation of molecular methods as alternatives to existing standard methods to help ensure the production of defendable results
• Highlights potential issues with respect to successful implementation of these methods

Spis treści

1. Food industry current status
2. Future directions for molecular microbial diagnostic methods for the food industry
3. Current status of molecular microbiological techniques for the analysis of drinking water
4. Future directions for molecular microbial diagnostic methods for the water industry 
5. EN ISO Standards for both culture and molecular methods
6. Laboratory validation, verification and accreditation of molecular methods
7. DNA extraction: finding the most suitable method
8. Assessing organism viability and interpreting genomic unit vs. colony forming unit data for water and food borne microorganisms such as Legionella, Campylobacter, Salmonella and Listeria
9. MALDI-TOF: A rapid microbiological confirmation technique for food and water analysis
10. Chapter highlights, future requirements and conclusions