Molecular Endocrinology: Methods and Protocols

ISBN-13: 9781603273770 / Angielski / Twarda / 2009 / 430 str.

O. Park-Sarge; Ok-Kyong Park-Sarge; JR. Thomas E. Curry

Opis

Over the course of the last hundred years, the field of endocrinology has received ever-increasing attention. Research and developing technologies have resulted in a significant leap in our understanding of the causes and treatment of endocrine dysfunction. In Molecular Endocrinology: Methods and Protocols, leaders in the field share a diversity of cutting-edge techniques that are becoming routinely employed in the quest to further understand hormone action. In order to reach the widest number of labs, this volume includes protocols that allow investigators at all stages of their scientific career to successfully perform these techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Molecular Endocrinology: Methods and Protocols is an ideal guide for researchers in labs across the world who seek a deeper understanding of the complex processes and biology of hormones."

Spis treści

1. Determining the Affinity of Hormone-Receptor Interaction David Puett and Krassimira Angelova 2. Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques Carole Bryant, John Moore, and Thomas E. Curry, Jr. 3. Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and their Functional Consequences at the Hormone-Receptor Interaction Level Evangelia Charmandari, George P. Chrousos, and Tomoshige Kino 4. Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane Mary Osisami, Huiyan Huang, and Michael A. Frohman 5. Gene Expression Profiling in the Aging Ovary Kathleen M. Eyster and John D. Brannian 6. Genomics Analysis: Endometrium Ricardo Francalacci Savaris and Linda C. Giudice 7. Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization Katherine L. Rosewell and Thomas E. Curry, Jr. 8. Adenoviral Gene Transfer into Isolated Pancreatic Islets Latha Muniappan and Sabire Özcan 9. Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes Anelia Horvath and Constantine Stratakis 10. Methylated DNA Immunoprecipitation and Microarray-based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines Yu-I Weng, Tim H.-M. Huang, Pearlly S. Yan 11. Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells Jingjing L. Kipp and Kelly E. Mayo 12. Use of Reporter Genes to Study Promoters of the Androgen Receptor Lirim Shemshedini 13. Isolation of Proteins Associated with the DNA-bound Estrogen Receptor a Jennifer R. Schultz-Norton, Yvonne S.Ziegler, Varsha S. Likhite, and Ann M. Nardulli 14. Chromosome-Wide Analysis of Protein Binding and Modifications Kevin D. Sarge, Hongyan Xing, and Ok-Kyong Park-Sarge 15. Genome-Wide Analysis for Protein-DNA Interaction: ChIP- Chip Yunguang Tong and Jeff Falk 16. Detection of ERa-SRC-1 interactions using Bioluminescent Resonance Energy Transfer Tamika Tyson Duplessis, Kristen L. Koterba, and Brian G. Rowan 17. Detection of Proteins Sumolyated In Vivo and In Vitro Kevin D. Sarge and Ok-Kyong Park-Sarge 18. Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE) Oladapo Yeku, Elizabeth Scotto-Lavino, and Michael A Frohman 19. Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis Sachiko Matsuzaki, Michel Canis, and Gérard Mage 20. Reporter Mice for the Study of Intracellular Receptor Activity Adriana Maggi and Gianpaolo Rando 21. Real-Time Non-Invasive Imaging of ES Cell-Derived Insulin Producing Cells Sudhanshu P. Raikwar and Nicholas Zavazava 22. Transgenic Mouse Technology: Principles and Methods T. Rajendra Kumar, Melissa Larson, Huizhen Wang, Jeff McDermott, and Illya Bronshteyn 23. Breast Tumor-Initiating Cells Isolated from Patient Core Biopsies for Study of Hormone Action Carolyn G. Marsden, Mary Jo Wright, Radhika Pochampally, and Brian G. Rowan 24. Markers of Oxidative Stress and Sperm Chromatin Integrity Ashok Agarwal, Alex C. Varghese, and Rakesh K. Sharma 25. Planning and Executing a Genome Wide Association Study (GWAS) Michèle M. Sale, Josyf C. Mychaleckyj, and Wei-Min Chen