Molecular Diagnostics of Cancer

ISBN-13: 9783540554769 / Angielski / Miękka / 1992 / 223 str.

Christoph Wagener; Siegfried Neumann

Opis

Present and potential future applications of new diagnostic strategies basedon the direct or indirect detection of cancer genes are delineated in this volume. Among the methodological aspects covered are enzymatic target amplification by the polymerase chain reaction and related techniques, DNA fingerprinting, transfer of putative cancer genes in appropriate receipient cells, and recent developments inthe application of monoclonal antibodies in immunohistochemistry and immunoscintigraphy. The diagnostic and functional implications of mutations in cancer genes such as ras and p53 are described. The characterization of cancer genes and their products is correlated with growth control anddissemination of tumour cells by in vitro or clinical evidence. The contributions in the present volume uptdate the information available on established or newly described cancer genes, and may help manage the transition from basic research to clinical practice.

Spis treści

The Polymerase Chain Reaction: Methodology and Diagnostic Potential.- Gene Loss as Marker in Tumor Development: Considerations on the Origin of Specific Brain Tumors Due to Combinant Loss of Function Mutations of Tumor Suppressor Genes.- p53: Oncogene, Tumor Suppressor, or Both?.- Tumor Genome Screening by Multilocus DNA Fingerprints as Obtained by Simple Repetitive Oligonucleotide Probes.- Activation of ras Oncogenes in Human Tumours.- Laboratory Techniques in the Investigation of Human Papillomavirus Infection.- Detection of Minimal Residual Leukemia by Polymerase Chain Reactions.- A Dominant Metastogene Confers Metastatic Potential to Tumor Cells.- Progression in Human Melanoma Is Accompanied by the Altered Expression of Cell Adhesion Molecules.- Structure, Function and Expression of the CEA Gene Family: Diagnostic and Therapeutic Implications.- Membrane Proteins as Markers for Normal and Neoplastic Endocrine Cells.- Biological and Clinical Relevance of the Tumor-Associated Serine Protease uPA.- Analysis of Growth Regulatory Pathways in Human Neuro-Oncology.- HER-2/Neu/c-ERB-B2 Amplification: Clinical Relevance in Mammary Carcinoma.- Cytokine-Mediated Regulation of Growth Factor Receptors (EGF-R and erb-B2) in Pancreatic Tumors.- Chimeric CEA-Specific Antibody Fragments and Anti-idiotypic Antibodies for Immunoscintigraphy and New Therapeutic Approaches to Colorectal Carcinomas.- Tumor Localization by Immunoscintigraphy: Potential and Limitations.