Molecular Dermatology: Methods and Protocols

ISBN-13: 9781071606476 / Angielski / Twarda / 2020 / 278 str.

​natalia V. Botchkareva; Gillian E. Westgate

Spis treści

1. Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration

            Irene Castellano-Pellicena and M. Julie Thornton

 

2. Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle

            Rachael Williams and M. Julie Thornton

 

3. Isolation of Epidermal and Hair Follicle Melanocytes

            Richard Baker and M. Julie Thornton

 

4. Isolation and Culture of Human Skin Mast Cells

            Hanna Siiskonen and Jörg Scheffel

 

5. Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines

            Gijs Rikken, Hanna Niehues, and Ellen H. van den Bogaard

 

6. Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts

            Isabelle Petit, Ayelet Levy, and Daniel Aberdam

 

7. A Method to Investigate the Epidermal Permeability Barrier In Vitro

            Yoshikazu Uchida and Anna Celli

 

8. Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion

            Summik Limbu and Claire A. Higgins

 

9. Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture

            Janin Lehmann, Jennifer Gherardini, and Marta Bertolini

 

10. Non-Surgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells

            Eddy H.C. Wang and Kevin J. McElwee

 

11. Collapse and Restoration of Hair Follicle Immune Privilege Ex Vivo: A Model for Alopecia Areata

            Markus Fehrholz and Marta Bertolini

 

12. Experimentally-Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo

            Ilaria Piccini and Marta Bertolini

 

13. Generation of Hair Follicle Germs in Vitro Using Human Postnatal Skin Cells

            Ekaterina P. Kalabusheva and Ekaterina A. Vorotelyak

 

14. Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micro-Punch Technique and Vital Dyes

            Francisco Jimenez

 

15. Identification of Long Non-Coding RNA by In Situ Hybridization Approaches

            Mara Mancini, Anna Maria Lena, and Eleonora Candi

 

16. Detection of MicroRNAs by In Situ Hybridization in Skin

            Maximilian E. Pickup and Mohammed I. Ahmed

 

17. Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells

            Carmit Bar, V. Julian Valdes, and Elena Ezhkova

 

18. 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells In Situ

            Andrei N. Mardaryev and Michael Y. Fessing

 

19. Method to Study Skin Cancer: Two-Stage Chemically-Induced Carcinogenesis in Mouse Skin

            Andrei N. Mardaryev

 

20. Investigation of Skin Wound Healing Using a Mouse Model

            Jianmin Wu and Ning Xu Landén

 

21. Human Wound Healing Ex Vivo Model with Focus on Molecular Markers

            Jennifer Gherardini, Max van Lessen, Ilaria Piccini, Janin Lehmann, and Marta Bertolini

 

22. Method for Investigation of Photobiological Effects of Light on Human Skin Cells Mediated by Low Doses of Light

            Charles Mignon and Natallia E. Uzunbajakava

Blurb

This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.