Molecular Chaperones: Methods and Protocols

ISBN-13: 9781617792946 / Angielski / Twarda / 2011 / 321 str.

Stuart K. Calderwood; Thomas L. Prince

Opis

The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In "Molecular Chaperones: Methods and Protocols," expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, "in vitro" and "in vivo." Written in the highly successful "Methods in Molecular Biology " series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, "Molecular Chaperones: Methods and Protocols" serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.

Recenzje

From the reviews:

"This book details current methods and protocols essential for understanding the biological properties and roles of the molecular chaperones ... . It appears to be written for all scientists interested in understanding the roles of molecular chaperones, which would include students and researchers interested in heat shock proteins. ... This will be a useful guide for all scientists interested in the field of heat shock proteins and molecular chaperones and their role in health and disease." (Omer Iqbal, Doody's Book Reviews, December, 2011)

Spis treści

1. Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

            Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi

 

2. The Role Of Heat Shock Factors in Stress-Induced Transcription     

            Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood

 

3. Hsp90 and Client Protein Maturation

            Natalie Wayne, Parul Mishra, and Daniel N. Bolon

 

4. The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function

            Marc B. Cox and Jill L. Johnson

 

5. Detecting Hsp90 Phosphorylation

            Mehdi Mollapour and Len Neckers

 

6. Role of Molecular Chaperones in Biogenesis of the Protein Kinome

            Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, and Avrom J. Caplan

 

7. Nucleotide Exchange Factors for Hsp70 Chaperones

            Heike Rampelt, Matthias P. Mayer, and Bernd Bukau

 

8. Reconstitution of CHIP E3 Ubiquitin Ligase Activity

            Hong Yu Ren, Cam Patterson, Douglas M. Cyr, and Meredith F.N. Rosser

 

9. Structure-Functions of HspB1 (Hsp27)

            André-Patrick Arrigo

 

10. Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene

            Punit Kaur, Ganachari M. Nagaraja, Khalid Bin Abdulrahman, and Alexzander Asea

 

11. Quantification of HSP27 and HSP70 Molecular Chaperone Activities

            Jessica Gobbo, Caroline Gaucher-Di-Stasio, Stéphanie Weidmann, Jean Guzzo, and Carmen Garrido

 

12. Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA

            H. Elyse Ireland and John H.H. Williams

 

13. Analysis of Heat Shock Protein Localization Using Flow Cytometry

            Nina C. Dempsey, Francesca Leoni, Christine Hoyle, and John. H.H. Williams

 

14. Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry

            Punit Kaur, Khalid Bin Abdulrahman, and Alexzander Asea

 

15. Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks

            Yunchen Gong, Zhaolei Zhang, and Walid A. Houry

 

16. Hsp70: Anti-Apoptotic and Tumorigenic Protein

            Anne-Laure Rérole, Gaëtan Jego, and Carmen Garrido

 

17. Determination of Cell Survival or Death

            Alexander  E. Kabakov, Vladimir A. Kudryavtsev, and Vladimir L. Gabai

 

18. Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer

            Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, and Alberto J.L. Macario

 

19. Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells

            Desheng Weng, Stuart K. Calderwood, and Jianlin Gong

 

20. Isolation of Heat Shock Protein Complexes

            Fernando D. Cuello-Carrión, Mariel A. Fanelli, Niubys Cayado-Gutiérrez, Gisela Castro, and Daniel R. Ciocca

 

21. Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein

            Xiang-Yang Wang, Huanfa Yi, Xiaofei Yu, Damin Zuo, and John R. Subjeck

 

22. Investigating Receptors for Extracellular Heat Shock Proteins

            Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood

 

23. Analysis of Cellular Migration Using a Two-Chamber Methodology

            Michelle M. Kouspou and John T. Price