Mitochondrial Bioenergetics: Methods and Protocols

ISBN-13: 9781493958375 / Angielski / Miękka / 2016 / 333 str.

Carlos M. Palmeira; Antonio J. Moreno

Opis

This Methods in Molecular Biology(TM) book offers practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Includes protocols, materials lists and tips.

Spis treści

1 - Overview of Mitochondrial Bioenergetics (Vitor M. C. Madeira)

2 – Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells (Ana M. Silva and Paulo J. Oliveira)

3 – High-Resolution Respirometry.  OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle (Dominik Pesta and Erich Gnaiger)

4 – High Throughput Analysis of Mitochondrial Oxygen Consumption (James Hynes, Rachel L. Swiss and Yvonne Will)

5 – Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices(Ana Ledo, Rui M. Barbosa and João Laranjinha)

6 - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria (Carlos M. Palmeira and Anabela P. Rolo)

7 - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells (Tiago R. Figueira, Daniela R. Melo, Aníbal E. Vercesi and Roger F. Castilho)

8 – Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells (D. Nicholls)

9 – Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria (Vilmante Borutaite and Rasa Baniene)

10 – Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function (Martin  Jastroch)

11 - Measurement of Proton Leak and Electron Leak in Isolated Mitochondria

 (Charles Affourtit, Casey L. Quinlan and Martin D. Brand)

12 - Relation between Mitochondrial Membrane Potential and ROS Formation (Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski and Mariusz R. Wieckowski)

 13 - Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport (António J. Moreno and Joaquim A. F. Vicente)

 14 - Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy (Sean M. Davidson and Michael R. Duchen)

 15 – Mitochondrial Permeability Transition Pore and Calcium Handling

 (Renee Wong, Charles Steenbergen and Elizabeth Murphy)

16- Imaging of Mitochondrial pH Using SNARF-1

(Venkat K. Ramshesh and John J. Lemasters)

17- Redox Equivalents and Mitochondrial Bioenergetics

(James R. Roede, Young-Mi Go and Dean P. Jones)

18-NMR Methodologies for Studying Mitochondrial Bioenergetics

(Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)

19-Computational Modeling of Mitochondrial Function

(Sonia Cortassa and Miguel A. Aon)

Blurb

Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.  These initiatives have fostered the investigation of genetic and environmental influences on bioenergetics. In Mitochondrial Bioenergetics: Methods and Protocols, researchers in the field detail the practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls

 

Authoritative and practical, Mitochondrial Bioenergetics: Methods and Protocols helps elevate the quality and rate of investigative discoveries regarding disease states associated with environmental or genetic influences on mitochondrial bioenergetics.