Mineralizing Vesicles: From Biochemical and Biophysical Properties to Their Roles in Physiology and Disease

ISBN-13: 9780323991582 / Angielski / Miękka / 2023

Massimo Bottini; Jose Luis Millan

Opis

Mineralizing Vesicles: From Biochemical and Biophysical Properties to Their Roles in Physiology and Disease presents the state-of-the art in the properties of mineralizing EVs and their potential clinical applications. The first chapter presents foundational biochemical and biological aspects of EVs. Next, the title coves the role of EVs in bone ossification and in cardiovascular and cartilage-related diseases. Considering the unique ability of this class of EVs to form apatite minerals assigned to their special biochemical machinery, three chapters of the book then focus on the enzymes catalysing the inorganic phosphate and calcium turn-over and the dynamic properties of the vesicles’ peripheral proteins. Chapters describe the role of inorganic phosphate and calcium ions and of autophagy on the biogenesis and function of mineralizing EVs. Recent studies show that the lumen of mineralizing EVs is partially filled with miRNA, and a chapter therefore considers research on the possible function of these vesicles as signalosomes. The final five chapters of the book describe practical aspects of working with mineralizing EVs, including their purification, proteomic and biophysical analyses, the use of biomimetic models and mineralizing EVs in regenerative medicine. This title presents, for the first time, a comprehensive account of mineralizing EVs and their potential clinical applications. It will be invaluable to researchers in the field.

Spis treści

1. General aspects of extracellular vesicles in bone formation and remodeling Antonio Maurizi and Anna Teti 2. Physiological biomineralization. Part I: the role of matrix vesicles in skeletal calcification Rene Buchet 3. Physiological biomineralization. Part II: the role of matrix vesicles in dental calcification Sandra Yasuyo Fukada 4. Physiological biomineralization. Part III: the role of intracellular vesicles and media vesicles Rene Buchet 5. Pathological biomineralization. Part I: Mineralizing EVs in cardiovascular diseases Saida Mebarek Sr. 6. Pathological biomineralization. Part II: Mineralizing EVs in osteoarthritis Ann K. Rosenthal 7. The biochemistry of mineralizing EVs. Part I: the role of phosphatases Jose Luis Millan 8. The biochemistry of mineralizing EVs. Part II: the role of annexins and phosphate transporters Slawomir Pikula 9. The role of calcium and phosphate in the release and function of mineralizing EVs Dobrawa Napierala 10. The role of autophagy in the release and function of mineralizing EVs Ana Paula Ramos 11. The roles of mineralizing EVs in cell-cell communication Yuji Yoshiko and Tomoko Minamizaki 12. Working with mineralizing EVs. Part I: purification techniques Rene Buchet 13. Working with mineralizing EVs. Part II: proteomic profiling Rene Buchet 14. Working with mineralizing EVs: Part III: biophysical characterization Massimo Bottini 15. Working with mineralizing. EVs: Part IV: biomimetic models of mineralizing extracellular vesicles Pietro Ciancaglini, Saida Mebarek Sr., Jose Luis Millan, Massimo Bottini, Ana Paula Ramos, Maytê Bolean, Heitor Sebinelli, Luiz Andrilli, Bruno Favarin, Ekeveliny Veschi, Marcos Cruz and Rene Buchet 16. Working with mineralizing EVs. Part V: use of mineralizing extracellular vesicles in bone regeneration Ana Paula Ramos