Microsystem Technology: A Powerful Tool for Biomolecular Studies

ISBN-13: 9783764357740 / Angielski / Twarda / 1999 / 581 str.

H. P. Saluz; J. M. Kohler; Hans-Peter Saluz

Opis

This book examines the rapidly evolving microsystem technology that promises to unravel a wide range of applications in biology, gene therapy, molecular medicine, and forensics. These applications will have crucial bearing on future work in the diagnosis of disease and combinatorial chemistry. In addition, the work covers application strategies, theoretical aspects, protocols, and patents.

Spis treści

1 Molecular building principles in nature.- 2 Robotic equipment and microsystem technology in biological research.- 3 Components and systems for microliquid handling.- 4 Nanotiterplates for screening and synthesis.- 5 Chip technology for micro-separation.- 6 Rapid multisample PCR in miniaturized ultrathin-walled microwell plates.- 7 Generation of large libraries.- 8 Generation and screening of solution-phase synthetic peptide combinatorial libraries.- 9 Using oligonucleotide probe arrays to access genetic diversity.- 10 Generation of libraries by print technologies.- 11 Synthesis and screening of bead-based libraries.- 12 Sensors for biomolecular studies.- 13 Fluorescence detection of single molecules applicable to small volume assays.- 14 Fluorescence correlation spectrometry (FCS): Measuring biological interactions in microstructures.- 15 Scanning force microscopy: A microstructured device for imaging, probing, and manipulation of biomolecules at the nanometer scale.- 16 DNA resequencing, mutation detection and gene expression analysis of oligonucleotide microchips.- 17 Miniaturized arrays for DNA analysis.- 18 Manipulation of particles, cells and liquid droplets by high frequency electric fields.- 19 Optical trapping and manipulation.- 20 Computer modeling of protein, nucleic acid, and drug structures.- 21 Optimizing structure and function relationship of nucleic acid molecules.- 22 Optimisation of molecular function.- 23 Appendix.