Microfluidic Electrophoresis: Methods and Protocols

ISBN-13: 9781493989638 / Angielski / Twarda / 2018 / 255 str.

Debashis Dutta

Spis treści

1. Fabrication of Glass Microfluidic Devices

            Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, and Shu Jia

 

2. Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices

            Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, and Sk Rameez Iqbal

 

3. Sample Injection Techniques

            James M. Karlinsey

 

4. Sample Preconcentration Protocols in Microfluidic Electrophoresis

            Fumihiko Kitagawa and Koji Otsuka

 

5. Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection

            Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, and José Alberto Fracassi da Silva

 

6. Micellar Electrokinetic Chromatography

            Braden C. Giordano, Ronald Seifert, and Greg E. Collins

 

7. Microchip Isotachophoresis: Analysis of Pharmaceuticals

            Marián Masár and Jasna Hradski

 

8. Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination

            Stefan Nagl

 

9. Nanochannel Gradient Separations

            Michael A. Startsev and David W. Inglis

 

10. Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications

            Cyro L.S. Chagas, Thiago M.G. Cardoso, and Wendell K.T. Coltro

 

11. Band Broadening Theories in Capillary Electrophoresis

            Sandip Ghosal

 

12. Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation

            Debashis Dutta

 

13. Microchip Electrophoresis Tools for the Analysis of Small Molecules

            Federico J.V. Gomez and María Fernanda Silva

 

14. Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out

            Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, and Frederic Zenhausern

 

15. Achieving Stable Electrospray Ionization  Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips

            Iulia M. Lazar

 

16. Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow

            Ling Xia and Debashis Dutta

Blurb

This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.