Microchip Capillary Electrophoresis: Methods and Protocols

ISBN-13: 9781588292933 / Angielski / Twarda / 2006 / 237 str.

Charles S. Henry

Opis

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Recenzje

"From this book the reader can acquire a basic command of the technique relatively fast and, if required, can move on to in-depth fundamental, or specific information in the literature of this explosively growing area of science." -Molecular Biotechnology

Spis treści

Part I. Microchip Fabrication Methods Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes Mark M. Crain, Robert S. Keynton, Kevin M. Walsh, Thomas J. Roussel, Jr., Richard P. Baldwin, John F. Naber, and Douglas J. Jackson Micro-Molding for Poly(dimethylsiloxane) Microchips Carlos D. García and Charles S. Henry Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation Laurie E. Locascio, David J. Ross, Peter B. Howell, and Michael Gaitan Part II. Surface Modification Methods Surface Modification Methods for Enhanced Device Efficacy and Function Barbara J. Jones and Mark A. Hayes Polyelectrolyte Coatings for Microchip Capillary Electrophoresis Yan Liu and Charles S. Henry Part III. Detection Methods for Microchip Capillary Electrophoresis Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry Trust Razunguzwa and Aaron T. Timperman Interfacing Amperometric Detection With Microchip Capillary Electrophoresis R. Scott Martin Conductivity Detection on Microchips Roland Hergenröder and Benedikt Graß Part IV. Applications of Microchip Capillary Electrophoresis DNA Separations Andrea W. Chow Protein Separations Andrea W. Chow Microchip Capillary Electrophoresis: Application to Peptide Analysis Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte Measuring Electroosmotic Flow in Microchips and Capillaries S. Douglass Gilman and Peter J. Chapman Single Cell Analysis on Microfluidic Devices Christopher T. Culbertson Rapid DNA Amplification in Glass Microdevices Christopher J. Easley, Lindsay A. Legendre, James P. Landers, and Jerome P. Ferrance Index

Blurb

Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Protocols, leading chemists and engineers concisely explain the principles behind this revolutionary technique and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. The authors describe in detail the detection methods used, including mass spectrometry, electrochemistry, and conductivity. Since surface chemistry must be carefully considered for optimal operation at this scale, they also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Instructive and well-detailed, Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for both initiating research in the field of microchip capillary electrophoresis and understanding the full range of how these new systems can be used successfully in biological research.