Microbial Lipid Production: Methods and Protocols

ISBN-13: 9781493994861 / Angielski / Miękka / 2020 / 422 str.

Venkatesh Balan

Opis

The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field.

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Microbial Lipid Alternatives to Plant Lipids
A. Daniel Joesn, Kyria L. Boundy-Mills, Florin Barla, Sandeep Kumar, Bryan Ubanwa, and Venkatesh Balan

2. Laboratory Screening Protocol to Identify Novel Oleaginous Yeasts
Irnayuli R. Sitepu, Antonio L. Garay, Tomas Cajka, Oliver Fiehn, and Kyria L. Boundy-Mills

3. Fungi (Mold) Based Lipid Production
Yan Yang, Fatemeh Heidari, and Bo Hu

4. Qualitative and Quantitative Estimation of Bacterial Lipid Production
Gangatharan Muralitharan, Manickam Gayathri, and Sumathy Shunmugam

5. Rhodococcus and Yarrowia Based Lipid Production using Lignin-Containing Industrial Residues
Rosemary K. Le, Kristina M. Mahan, and Arthur J. Ragauskas

6. Quantifiation of Lipid Content in Oleaginous Biomass using Thermogravimetry
Balakrishna Maddi, Agasteswar Vadlamani, Sridhar Viamajala, and Sasidhar Varanasi

7. Extraction and Characterization of Lipids from Macroalgae
David R. Nobles, Jr., and Schonna R. Manning

8. Genetic Engineering Approaches used to Increase Lipid Production and Alter Lipid Profile in Microbes
Xiao-Ling Tang and Ya-Ping Xue

9. Extraction Methods used to Separate Lipids from Microbes
Balaskrishna Maddi

10. Novel Microbial Modification Tools to Convert Lipids into Other Value Added Products
Priya Kumari, Farnaz Yusuf, and Naseem A. Gaur

11. Alkaline and Alkaline-Oxidative Pretreatment and Hydrolysis of Herbaceous Biomass for Growth of Oleaginous Microbes
Jacob D. Crowe, Muyang Li, Daniel L. Williams, Alex D. Smith, Tongjun Liu, and David B. Hodge

12. Conversion of Cultivated Oleaginous Organisms into Biocrude for Biofuel Applications
Eboibi Blessing, Umakanta Jena, and Senthil Chinnasamy

13. Life Cycle Analysis of Producing Microbial Lipids and Biodiesel: Comparison with Plant Lipid
Tom Bradley and Daniel Maga

14. Assessment of Fuel Quality Parameters and Selection of Bacteria using PROMETHEE-GAIA Algorithm
Sumathy Shunmugam, Manickam Gayathri, and Gangatharan Muralitharan

15. Production of High-Value Polyunsaturated Fatty Acids using Microbial Cultures
Mingjie Jin, Rui Zhai, Zhaoxian Xu, and Zhiqiang Wen

16. Screening for Oily Yeasts Able to Convert Hydrolyzates from Biomass to Biofuels While Maintaining Industrial Process Relevance
Patricia J. Slininger, P.J., Bruce S. Dien, Joshua C. Quarterman, Stephanie R. Thompson, and Cletus P. Kurtzman

17. Conversion of Microbial Lipids to Biodiesel and Basic Lab Tests for Analysis of Fuel-Quality Parameters
Annaliese K. Franz and Cody Yothers

18. Impact of Culture Conditions on Neutral Lipid production of Oleaginous Yeast
Irene Fakankun, Maryam Mirzaei, and David B. Levin

19. Producing Oleaginous Microorganisms using Wastewater: Methods and Guidelines for Lab and Industrial Scale Production
Kayla M. Rude, Tyler J. Barzee, and Annaliese K. Franz

20. Volatile Fatty Acid Production from Anaerobic Digestion of Organic Residues
Sibel Uludag-Demirer, Wei Liao, and Goksel N. Demirer

21. Producing Oleaginous Organisms using Food Waste: Challenge and Outcome
Singaram Jayanthi and Arun Kumar Thalla

22. Microbial Surfactants: Alternative to Vegetable Oil Surfactants
Eduardo Gudiña and Lígia R. Rodrigues

23. Evaluation of Bacterial Lipid Production: Quantitative and Qualitative Measurements – Tips and Guidelines
Sima Modiri, Hossein Shahbani Zahiri, and Kambiz Akbari Noghabi

24. Production of Oleaginous Organisms or Lipids using Sewage Water and Industrial Waste Water
Farha Deeba, Vikas Pruthi, and Yuvraj S. Negi