Microarrays in Clinical Diagnostics

ISBN-13: 9781588293947 / Angielski / Twarda / 2005 / 288 str.

Thomas O. Joos; Thomas O. Joos; Paolo Fortina

Opis

Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls."

Recenzje

From the reviews:

"a volume for the specialist with a fascination for the optimal collection and analysis of large data sets in microarray format."-SGM Quarterly

"...there is no other comparable book on the market...highly recommended to anyone interested in this rapidly evolving field of technology..." - Doody's Health Science Book Review Journal

"Microarray technology has become a mainstay of biological research. ... The book is divided into 15 chapters ... . Overall this book offers a good read to both clinicians and researchers at the bench who wish to enter the field of microarrays. The format of the book also provides a good roadmap to graduate students wishing to translate aspects of their research to methods amenable to microarrays." (Sridar V. Chittur, Clinical Chemistry, Vol. 52 (6), June, 2006)

"Microarrays in Clinical Diagnostics ... is a collection of fifteen chapters by authors from both industry and academia, surveying current applications of microarray-based technologies in diagnostics ... . provides protocols in the successful style of the Methods in Molecular Biology series, with clarity and sufficient technical detail so that both novice and current microarray users are sure to benefit from this book." (Nicolas Winssinger, ChemMedChem, Issue 1, 2006)

Spis treści

Noncontact Laser Microdissection and Pressure Catapulting: Sample Preparation for Genomic, Transcriptomic, and Proteomic Analysis Yilmaz Niyaz, Monika Stich, Bernd Sägmüller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer, Rainer Gangnus, and Karin Schütze Applications of the Universal DNA Microarray in Molecular Medicine Reyna Favis, Norman P. Gerry, Yu-Wei Cheng, and Francis Barany Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization Zhi-wei Zhang, Yi-ming Zhou, Yan Zhang, Yong Guo, Sheng-ce Tao, Ze Li, Qiong Zhang, and Jing Cheng Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays Lovisa Lovmar and Ann-Christine Syvänen Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology Maurizio Ferrari, Laura Cremonesi, Pierangelo Bonini, Barbara Foglieni, and Stefania Stenirri Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader® Margaret A. Keller Controlled Agitation During Hybridization: Surface Acoustic Waves Are Shaking Up Microarray Technology Achim Wixforth Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Regulator Gene by Luminex® xMAP™ Suspension Array Sherry A. Dunbar and James W. Jacobson Protein Microarray-Based Screening of Antibody Specificity Rhonda Bangham, Gregory A. Michaud, Barry Schweitzer, and Paul F. Predki Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection Brian B. Haab Allergen Microarrays Tito Bacarese-Hamilton, Julian Gray, Andrea Ardizzoni, and Andrea Crisanti Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples Ruochun Huang, Qian Shi,Weimin Yang, and Ruo-Pan Huang Multiplexed Cytokine Sandwich Immunoassays: Clinical Applications Uma Prabhakar, Edward Eirikis, Bruce E. Miller, and Hugh M. Davis Validation and Quality Control of Protein Microarray-Based Analytical Methods Larry J. Kricka and Stephen R. Master Tissue Microarrays Ronald Simon, Martina Mirlacher, and Guido Sauter Index

Blurb

Microarray technology is now well established in laboratories all over the world, both for rapid detection of genetic mutations and, increasingly, for simultaneously analyzing the expression of a large number of proteins. In Microarrays in Clinical Diagnostics, a panel of academic and industrial investigators surveys the new world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification and quantification of proteins, and for studies of protein-protein interactions. Among the highlights are universal arrays, antibody microarrays, direct labeling approaches, and quality control techniques for protein microarray-based analysis. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Microarrays in Clinical Diagnostics combines a comprehensive collection of cutting-edge technologies with detailed laboratory methods to help clinical laboratory researchers design and perform microarray experiments for DNA and protein analysis, diagnostic testing, and cancer research.